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用１１种培养基，即ＣＭＡ，ＴＳＡ，ＰＤＡ，ＰＳＡ，ＮＡ，ＫＭＢ，ＷＳＡ，ＷＡ，ＯＭＡ，ＶＤＹＡ和ＤＰＹＡ，在培养皿平板上，２８℃培养下，测定它们对西瓜枯萎病菌拮抗细菌筛选能力。由西瓜皮提取汁，蔗糖，溴麝香草酚蓝和琼脂配制成的ＷＳＡ培养基在４ｄ内可清晰地分辨出拮抗细菌，ＴＳＡ培养基广泛地用于测定来自作物根部对根部病原真菌具有拮抗作用的拮抗细菌，但与ＷＳＡ培养基比较，在其上细菌和真菌生长较弱，较不明显，因而只能较低水平地检测出拮抗细菌。在ＣＭＡ和ＷＡ培养基上，细菌生长最慢，有些种类的细菌甚至在ＷＡ培养基上不能生长。在ＰＤＡ，ＰＳＡ和ＯＭＡ培养基上ＦＯＮ菌落生长很快，因而不能检出抑制区域，而在ＮＡ、ＰＤＡ和ＫＭＢ培养基上某些细菌菌落生长很快，甚至与ＦＯＮ菌落相重叠，故不能出现抑菌圈，在ＶＤＹＡ和ＤＰＹＡ上ＦＯＮ和某些细菌生长相对较慢。
They were tested for their antagonistic bacterial screening ability against Fusarium oxysporum in 11 culture media, ie CMA, TSA, PDA, PSA, NA, KMB, WSA, WA, OMA, VDYA and DPYA on a culture dish plate at 28 ° C . WSA medium prepared from watermelon peel juice, sucrose, bromothymol blue and agar was able to clearly distinguish antagonistic bacteria within 4 days. TSA medium was widely used to determine the antagonism of root pathogens from crop roots However, compared with WSA medium, the growth of bacteria and fungi was weaker and less obvious, so only antagonistic bacteria could be detected at lower levels. Bacteria grow most slowly on CMA and WA media, and some species of bacteria do not grow on WA media. FON colonies grew rapidly on PDA, PSA, and OMA media, and thus could not detect the inhibitory zone, whereas some bacterial colonies on NA, PDA, and KMB medium grew rapidly, even overlapping with FON colonies, and therefore could not occur Zone of inhibition, FON and some bacteria grow relatively slowly on VDYA and DPYA.