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采用敏感的聚合酶链反应(PCR),测定急性淋巴细胞白血病(ALL)、儿童脑脊液(CSF)、肿瘤性淋巴细胞、T-细胞受体δ(TCRD),基因的克隆重排,以发现CSF中仅有少数白血病细胞,用免疫方法尚难诊断的中枢神经系统(CNS)白血病。 材料和方法 取新近被诊断为ALL21例儿童(年龄在1.5～14.5岁间)骨髓和CSF样本,ALL按FAB标准和免疫分型诊断。无CNS累及的细胞形态学证据。污染红细胞的CSF样本不作PCR检查。按标准方法从骨髓或外周血提取DNA备用。引物核苷酸序列为Vδ1,Vδ2,Vδ3,Dδ2.1,Dδ2.11,Dδ3.1,Dδ3.11,Jδ1等。
Sensitive polymerase chain reaction (PCR) was used to determine the clonal rearrangements of acute lymphoblastic leukemia (ALL), children’s cerebrospinal fluid (CSF), neoplastic lymphocytes, T-cell receptor δ (TCRD) In only a few leukemic cells, immunohistochemistry is still difficult to diagnose central nervous system (CNS) leukemia. Materials and Methods Bone marrow and CSF samples from 21 newly diagnosed ALL children (aged 1.5-14.5 years) were diagnosed ALL according to FAB criteria and immunophenotyping. No CNS involvement of cell morphology evidence. CSF samples contaminated with erythrocytes were not subjected to PCR. DNA is extracted from bone marrow or peripheral blood according to standard methods. Primer nucleotide sequence Vδ1, Vδ2, Vδ3, Dδ2.1, Dδ2.11, Dδ3.1, Dδ3.11, Jδ1 and so on.