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目的:建立独一味水提物、水洗脱物和70%乙醇溶液洗脱物中总苯乙醇苷含量的测定方法。方法:加独一味药材质量18倍量水,煎煮提取3次,每次1.5 h,滤过,合并滤液,滤液2~4℃冷藏除尘,60℃减压干燥得到水提物,水提物经聚酰胺和大孔树脂富集分离,分别用水和70%乙醇溶液洗脱,分别得到水洗脱物,70%乙醇溶液洗脱物Ⅰ(通过聚酰胺柱洗脱物)和70%乙醇溶液洗脱物Ⅱ(通过大孔树脂柱洗脱物);在224,252,256,354 nm波长处,采用一阶导数紫外分光光度法测定总苯乙醇苷的含量。结果:建立的一阶导数紫外分光光度法测定总苯乙醇苷的含量,在354 nm波长处加样回收率99.71%,RSD 2.5%。结论:该方法专属性强、灵敏度高、精确度与重复性好,适用于独一味中总苯乙醇苷的含量测定。
OBJECTIVE: To establish a method for the determination of total phenylethanoid glycosides in water extracts, water extracts and 70% ethanol solution. Method: Add 18 times the amount of water, decoction extract three times each time, each time 1.5 h, filtration, the combined filtrate, the filtrate 2 ~ 4 ℃ cold storage, drying under reduced pressure at 60 ℃ to obtain water extract, water extract Polyamide and macroporous resin were separated by concentration and eluted with water and 70% ethanol solution respectively to obtain water eluate, 70% ethanol solution eluate I (through polyamide column eluate) and 70% ethanol solution Eluate II (eluted through the macroporous resin column); at the wavelength of 224,252,256,354 nm, the first derivative UV spectrophotometric determination of total phenylethanoid glycosides. Results: The first derivative UV spectrophotometry was used to determine total phenylethanoid glycosides. The average recovery was 99.71% and RSD 2.5% at 354 nm. Conclusion: This method is specific, sensitive, accurate and reproducible. It is suitable for the determination of total phenylethanoid glycosides in the only flavor.