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将雄性SD大鼠分为对照组、1.5 mg·kg~(-1)PM_(2.5)组、5.6 mg·m-3SO_2组及1.5、6、24 mg·kg~(-1)PM_(2.5)和5.6 mg·m-3SO_2联合作用组.采用HE染色法、荧光实时定量PCR和ELISA等方法测定各组大鼠心脏组织病理学变化和炎症因子IL-1β、IL-6、TNF-α、i NOS基因表达及NO含量.结果表明,与对照组相比,SO_2(5.6 mg%m-3)或PM_(2.5)(1.5 mg·kg~(-1))单独作用没有引起明显的心肌细胞损伤,而PM_(2.5)和SO_2共同作用导致不同程度的心肌排列紊乱,细胞间隙增大及炎症细胞浸润和出血,比SO_2(5.6 mg%m-3)或PM_(2.5)(1.5 mg·kg~(-1))单独作用有严重的病理学损伤.1.5mg·kg~(-1)PM_(2.5)和SO_2引起4个基因表达和NO水平的变化与对照组相比没有统计学意义,而PM_(2.5)和SO_2联合处理引起大鼠心脏炎症因子表达水平比对照组显著增高,同时与SO_2组或PM_(2.5)组相比也有显著升高.提示在本实验条件下,相比于PM_(2.5)(1.5 mg·kg~(-1))和SO_2(5.6 mg·m-3)单独作用,二者复合暴露能引起心脏组织病理损伤和炎症因子高表达,这可能是PM_(2.5)和SO_2引发心脏疾病的重要机制.
Male SD rats were divided into control group, 1.5 mg · kg -1 PM 2.5 group, 5.6 mg · m -3 S 2 group and 1.5, 6, 24 mg · kg -1 PM 2.5, And 5.6 mg · m-3SO_2 groups.Hematoxylin-eosin staining, real-time quantitative PCR and ELISA were used to determine the changes of cardiac pathology and the expressions of IL-1β, IL-6 and TNF-α NOS gene expression and NO content.The results showed that SO 2 (5.6 mg% m-3) or PM 2.5 (1.5 mg · kg -1) alone did not cause significant cardiomyocyte injury compared with the control group (2.5 mg · kg -1) and PM 2.5 (-2.5 mg · kg -1), while the combination of PM 2.5 and SO 2 led to different degrees of myocardial disarrangement, cell gap enlargement and inflammatory cell infiltration and bleeding, (-1)) had serious pathological injury alone.No significant difference was observed in the expression of four genes and the NO level induced by 1.5 mg · kg -1 PM 2.5 and SO 2 compared with the control group Compared with control group, PM_ (2.5) and SO_2 combined treatment significantly increased cardiac inflammatory cytokines expression, compared with SO_2 group or PM_ (2.5) group also significantly increased, suggesting that under the experimental conditions, compared with PM_ (2.5) (1.5 mg · kg -1) and SO 2 (5.6 mg · m- 3) alone, the combination of the two can cause pathological damage of heart tissue and high expression of inflammatory cytokines, which may be the important mechanism of PM_ (2.5) and SO_2-induced heart disease.