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目的:建立复方丹参片中三七皂苷R_1,人参皂苷Rg_1及人参皂苷Rb_1含量的方法。方法:采用HPLC-ELSD方法,Agilent ODS C_(18)柱(250 mm×4.6 mm,5μm),流动相为乙腈-水,梯度洗脱,ELSD为栓测器,载气为氮气流速2.0 L·min~(-1),漂移管温度70℃。结果:三七中皂苷R_1,人参皂苷Rg_1及人参皂苷Rb_1的线性范围分别为:0.21～2.14μg(r=0.9998),1.0～10.0μg(r=0.999 3)和0.93～9.28μg(r=0.9991),平均回收率(n=5)分别105.7%(RSD 2.0%),100.7%(RSD 1.2%)和101.4%(RSD1.2%)。结论:方法简单,快速和准确,用于复方丹参片的质量控制。
OBJECTIVE: To establish a method for the content of notoginsenoside R_1, ginsenoside Rg_1 and ginsenoside Rb_1 in compound Danshen tablets. Methods: HPLC-ELSD method was used on Agilent ODS C18 column (250 mm×4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-water with gradient elution. ELSD was used as the detector. The flow rate of carrier gas was 2.0 L·. Min~(-1), drift tube temperature 70°C. Results: The linear ranges of ginsenoside R_1, ginsenoside Rg_1 and ginsenoside Rb_1 in Panax notoginseng were 0.21 to 2.14 μg (r=0.9998), 1.0 to 10.0 μg (r=0.999 3) and 0.93 to 9.28 μg (r=0.9991, respectively). The average recovery rate (n=5) was 105.7% (RSD 2.0%), 100.7% (RSD 1.2%) and 101.4% (RSD 1.2%), respectively. Conclusion: The method is simple, rapid and accurate. It is used for quality control of compound Danshen tablets.