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目的:通过研究对乙酰氨基酚合用琥乙红霉素对大鼠肝药物代谢酶的影响,对二者合用后的有效性和安全性作出评价,为临床合理用药提供理论依据。方法:采用CO还原差示光谱法,检测细胞色素P450(CYP450)及细胞色素b5(Cytb5)的含量;采用紫外-可见光分光光度法测定谷胱甘肽转移酶(GST)、红霉素N-脱甲基酶(ERD)和氨基比林N-脱甲基酶(ADM)的活性。结果:对乙酰氨基酚合用琥乙红霉素对大鼠肝微粒体CYP450的含量和活性有不同程度的影响。结论:对乙酰氨基酚合用琥乙红霉素对药物代谢酶有影响,可能使药物的体内代谢特征发生改变,产生药物代谢性相互作用,从而影响药物疗效增加肝脏损伤。
OBJECTIVE: To study the effect of paracetamol combined with erythromycin ethylsuccinate on hepatic drug metabolizing enzymes in rats and to evaluate the efficacy and safety of the combination of the two drugs in order to provide a theoretical basis for clinical rational drug use. Methods: The contents of cytochrome P450 (CYP450) and cytochrome b5 (Cytb5) were detected by CO reduction spectrophotometry. The contents of glutathione transferase (GST), erythromycin N- Demethylase (ERD) and aminopyrine N-demethylase (ADM) activity. Results: Paracetamol combined with erythromycin ethylsuccinate on rat liver microsomal CYP450 content and activity to varying degrees. Conclusion: Paracetamol combined with erythromycin ethylsuccinate on drug metabolizing enzymes may affect the metabolism of drugs in vivo changes in the body, resulting in drug-metabolic interactions, thus affecting the efficacy of drugs to increase liver damage.