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目的探讨不同因子对罗勒Ocimum basilicum发根生长及次生代谢产物的影响。方法用发根农杆菌1025感染罗勒叶片,建立发根培养体系,并利用紫外分光光度法测定不同培养时间及培养基中添加激素和诱导子后罗勒发根中多糖和总黄酮的量。结果激动素(KT)为罗勒发根培养的最适外源激素;茉莉酸甲酯(MeJA)对罗勒发根的生长有抑制作用,但明显促进总黄酮的形成;水杨酸(SA)对罗勒发根的生长影响不显著,但对多糖和总黄酮的积累有明显促进作用。结论 5种外源激素和2种诱导子均能不同程度地影响罗勒发根的生长及次生代谢产物的积累,为进一步筛选适宜的罗勒发根培养系统并调控次生代谢产物的研究奠定了基础。
Objective To investigate the effects of different factors on root growth and secondary metabolites of Ocimum basilicum. Methods Agrobacterium rhizogenes 1025 was used to infect the leaves of basil. The hairy root culture system was established. The amount of polysaccharides and total flavonoids in basal roots of basil were determined by UV spectrophotometry with different culture time and hormone and elicitor added. Results KT was the most suitable exogenous hormone for rooting in basil. MeJA inhibited the growth of basilar hairy roots and promoted the formation of total flavonoids. Salicylic acid (SA) The root growth of basil had no significant effect on the growth of the roots, but it significantly promoted the accumulation of polysaccharides and total flavonoids. CONCLUSION Five kinds of exogenous hormones and two kinds of elicitors can affect the root growth and the accumulation of secondary metabolites in basil to varying degrees, which laid the foundation for the further screening of appropriate basal hairy root culture system and regulation of secondary metabolites basis.