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目的探讨单核/巨噬细胞趋化因子在新生儿缺氧缺血性脑病(HIE)发病机制中的作用,为进一步治疗和预防新生儿HIE提供理论依据。方法收集2003年4月至2004年5月缺氧缺血性脑病新生儿34例,分为轻度脑病组,中、重度脑病组,分别于生后24h、72h及7d取静脉血应用ELISA方法测定其血清MCP-1值,对照组20例,生后24h取血化验,同样方法测定血清MCP-1值。结果HIE组患儿血清单核细胞趋化蛋白-1(MCP-1)及巨噬细胞炎性蛋白-1α(MIP-1α)值明显高于对照组(P<0.05);中、重度HIE患儿血清MCP-1及MIP-1α值明显高于轻度HIE患儿(P<0.01),且生后72h仍保持较高水平,与对照组比较差异有统计学意义(P<0.05);血清MCP-1及MIP-1α值具有正相关性(P<0.05)。结论单核/巨噬细胞趋化因子参与了HIE的发病过程,且病情越重,趋化因子值越高,提示MCP-1及MIP-1α作为趋化性细胞因子在缺氧缺血性脑损伤过程中发挥了重要的作用,进一步研究MCP-1及MIP-1α的生物学功能及其调节信号转导机制,为预防和治疗HIE开辟新的思路。
Objective To investigate the role of monocyte / macrophage chemokine in the pathogenesis of neonatal hypoxic-ischemic encephalopathy (HIE) and to provide a theoretical basis for further treatment and prevention of neonatal HIE. Methods From April 2003 to May 2004, 34 newborn infants with hypoxic ischemic encephalopathy were divided into mild encephalopathy group, moderate and severe encephalopathy group, and venous blood was collected at 24h, 72h and 7d after birth by ELISA The serum MCP-1 level was measured, 20 cases in the control group and 24 hours after birth, and the serum MCP-1 level was measured by the same method. Results The levels of MCP-1 and MIP-1α in HIE group were significantly higher than those in control group (P <0.05). The moderate and severe HIE patients Serum MCP-1 and MIP-1α levels were significantly higher in children with mild HIE than in those with mild HIE (P <0.01), and maintained at a high level at 72 hours after birth, which was significantly different from the control group (P <0.05) There was a positive correlation between MCP-1 and MIP-1α (P <0.05). Conclusions Monocyte / macrophage chemotactic factor is involved in the pathogenesis of HIE, and the more serious the disease, the higher the chemokine value, suggesting that MCP-1 and MIP-1α as chemoattractant cytokines in hypoxic-ischemic brain Play an important role in the process of injury, to further study the biological function of MCP-1 and MIP-1α and its regulation of signal transduction mechanism, to open up new ideas for the prevention and treatment of HIE.