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Objecive To check and compare the expression levels of human androgen receptor (AR) mRNA in both normal adult prostate (NAP) and benign prostate hyperplasia (BPH) tissues, and to try to find if BPH results from the abnormal transcription of ARmRNA in prostate. Methods Expression of the human ARmRNA in 14 paraffin-embedded prostate tissues (4 cases of NAP and 10 cases of BPH) was studied with the direct in situ reverse transcription-polymerase chain reaction (RT-PCR). Quantitative analysis of the ARmRNA products was performed using the image analysis system. Results ①Specific ARmRNA was detected in both NAP and BPH specimens and in both epithelia and interstitial cells. The positive products were relatively densely localized in the cytoplasm of perinuclear zone. ② The intensity of ARmRNA signals in epithelial cells was significantly stronger than that in interstitial cells (P<0.001). However, there was no statistically significant difference in ARmRNA level between NAP and BPH; ③ The heterogeneity of ARmRNA signal and the androgen-independent cells were observed in prostatic epithelia. Stronger positive signals of ARmRNA were shown in a few basal-cell layer (BCL) cells of BPH tissue, but were not found in that of NAP tissue. Conclusion Results of this study show that there is no significant difference in the ARmRNA expression between NAP and BPH groups in both epithelium and interstitial cells. It may indicate that BPH does not result from the ARmRNA transcription in the prostate.
Objecive To check and compare the expression levels of human androgen receptor (AR) mRNA in both normal adult prostate (NAP) and benign prostate hyperplasia (BPH) tissues, and to try to find if BPH results from the abnormal transcription of AR mRNA in prostate. Methods Expression of the human AR mRNA in 14 paraffin-embedded prostate tissues (4 cases of NAP and 10 cases of BPH) was studied with the direct in situ reverse transcription-polymerase chain reaction (RT-PCR). Quantitative analysis of the AR mRNA products was Results ① Specific AR mRNA was detected in both NAP and BPH specimens and in both epithelia and interstitial cells. The positive products were relatively densely localized in the cytoplasm of perinuclear zone. ② The intensity of AR mRNA signals in epithelial cells was Significally stronger than that in interstitial cells (P <0.001). However, there was no statistically significant difference in ARmRNA level between NAP and BPH; The heterogeneity of ARmRNA signal and the androgen-independent cells were observed in prostatic epithelia. Stronger positive signals of AR mRNA were shown in a few basal-cell layer (BCL) cells of BPH tissue, but were not found in that of NAP tissue. Results of this study show that there is no significant difference in the ARmRNA expression between NAP and BPH groups in both epithelium and interstitial cells. It may indicate that BPH does not result from the AR mRNA transcription in the prostate.