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目的：探讨ｋｉ－６７抗原表达与胃癌的恶性生物学行为，特别是与远隔脏器转移行为的关系。方法：应用ＤＡＫＯ公司新推出的兔抗人ｋｉ－６７多克隆抗体，对５６例胃癌的常规病理切片进行ＡＢＣ免疫组化检测。结果：ｋｉ－６７抗原表达量与胃癌组织学类型、分化程度、生长方式、胃壁浸润深度以及区域淋巴结转移无明显相关（Ｐ＞０．０５）。与胃癌的肝、卵巢、肾上腺等远隔脏器的转移呈明显正相关（Ｐ＜０．００５）。ｋｉ－６７抗原表达量与胃癌组织的ＤＮＡ异倍体呈明显正相关（Ｐ＜０．０５）。结论：ｋｉ－６７抗原作为一个代表增殖活性的肿瘤标志，其过量表达与胃癌细胞的恶性生物学行为，特别是与远隔脏器转移行为密切相关，故可应用于临床以准确估计胃癌的预后和判断远隔脏器转移的危险性
Objective: To investigate the expression of ki-67 antigen and the malignant biological behavior of gastric cancer, especially the relationship with distant metastasis. Methods: A rabbit anti-human ki-67 polyclonal antibody was introduced from DAKO company. ABC immunohistochemistry was performed on routine pathological sections of 56 cases of gastric cancer. RESULTS: The expression of ki-67 antigen was not significantly associated with the histological type, differentiation, growth pattern, infiltration depth of gastric wall and regional lymph node metastasis (P>0.05). There was a significant positive correlation between the distant metastases of the liver, ovary, and adrenal glands of gastric cancer (P<0.005). The expression of ki-67 antigen was significantly positively correlated with DNA aneuploidy in gastric cancer tissues (P<0.05). Conclusion: ki-67 antigen is a tumor marker that represents the proliferative activity. Overexpression of ki-67 antigen is closely related to the malignant biological behavior of gastric cancer cells, especially to distant organ metastasis. Therefore, it can be used clinically to accurately estimate the prognosis of gastric cancer. And judging the risk of distant organ transfer