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许多作者从染色体畸变和粉碎化方面报道了病毒引起的遗传性损伤。此外还观察到大量的病毒诱发姐妹染色单体交换(SCE),虽然诱发的程度不相同。本研究试图测定脊髓灰质炎患者的SCE和染色体畸变的频率。选择25名年龄相近的脊髓灰质炎患者。对照组为20名在近期内未服用过任何药物并未受病毒感染的健康者。在作出临床诊断6星期后,患者尚未服药前采集血样,做两组全血培养。将BrdU加入一组培养基中,最终浓度为10μg/ml。37℃培养48小时后收获标本,气干
Many authors report genetic damage caused by viruses from chromosomal aberrations and shredding. In addition, a large number of viruses were also observed to induce sister chromatid exchange (SCE), albeit to a different extent. This study sought to determine the frequency of SCE and chromosomal aberrations in patients with poliomyelitis. Twenty-five polio patients of similar age were selected. The control group consisted of 20 healthy individuals who had not taken any medication in the near future and had not been infected with the virus. Six weeks after making a clinical diagnosis, the patient has not taken a blood sample before taking the medication and does two whole blood cultures. BrdU was added to a group of media at a final concentration of 10 μg / ml. After 48 hours of culture at 37 ℃, the specimens were harvested and air-dried