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目的:探讨草苁蓉提取物(BRE)联合长春瑞滨(NVB)化疗对兔VX2移植瘤的治疗作用和抗氧化能力的影响。方法:建立兔VX2皮下移植瘤模型,将其分为模型组,BRE组,NVB组和BRE+NVB组(BRE和NVB联合组)。于造模第8天开始给药,BRE组和BRE+NVB组分别ig给药100 mg.(kg.d)-1 BRE共21 d,NVB组和BRE+NVB组于造模第15,22天静脉注射2.5 mg·kg-1.d-1 NVB共2次。观察各组荷瘤兔的肿瘤生长情况。实验结束后,称瘤重和计算抑瘤率。提取瘤体标本,免疫组化法检测移植瘤细胞增殖细胞核抗原(PCNA)表达情况。比色法检测血清超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性及总抗氧化能力(TAOC)和丙二醛(MDA)含量。结果:各治疗组对移植瘤生长和肿瘤细胞增殖有明显的抑制作用,其中BRE+NVB组肿瘤生长抑制率最高、增殖指数最小。同时,NVB虽明显增高血清CAT,GSH-Px活性,但SOD活性和TAOC水平显著降低,MDA水平明显增高。而BRE组和BRE+NVB组均可明显升高血清SOD,CAT,GSH-Px活性和TAOC水平,降低MDA水平。结论:BRE对VX2移植瘤具有明显的抑制作用,且对长春瑞滨化疗有明显增强作用。其作用可能与其抑制细胞增殖和增高机体抗氧化能力有关。
Objective: To investigate the effects of BRE combined with vinblastine (NVB) on the therapeutic effect and antioxidant capacity of VX2 tumor in rabbits. Methods: Rabbit VX2 subcutaneously transplanted tumor model was established and divided into model group, BRE group, NVB group and BRE + NVB group (BRE and NVB combined group). The rats in the BRE group and the BRE + NVB group were dosed with 100 mg. (Kg · d -1) BRE for 21 days, and those in the NVB group and the BRE + NVB group Intravenous injection of 2.5 mg · kg-1.d-1 NVB 2 times. Tumor growth in each group was observed. After the experiment, said tumor weight and calculate the inhibition rate. Tumor specimens were extracted and the expression of proliferating cell nuclear antigen (PCNA) was detected by immunohistochemistry. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and total antioxidant capacity (TAOC) and malondialdehyde )content. Results: The treatment groups significantly inhibited the growth of tumor xenografts and the proliferation of tumor cells. The tumor growth inhibition rate and the proliferation index of BRE + NVB group were the lowest. At the same time, NVB significantly increased serum CAT, GSH-Px activity, but SOD activity and TAOC levels were significantly reduced, MDA levels were significantly increased. But BRE group and BRE + NVB group could significantly increase serum SOD, CAT, GSH-Px activity and TAOC level, reduce MDA level. Conclusion: BRE has a significant inhibitory effect on VX2 xenografts and a marked enhancement of vinorelbine chemotherapy. Its role may be related to its inhibition of cell proliferation and increase the body’s antioxidant capacity.