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神经系统变性疾病(ND)的病理特征是蛋白质聚集在细胞内或细胞外形成异常的堆积体而导致神经毒性。双分子荧光互补技术(Bi FC)是研究ND相关蛋白质寡聚化分子机制的重要工具。与其他技术不同的是,Bi FC不但可以在生理环境中对蛋白质-蛋白质之间的相互作用进行可视化检测,而且能够提供详细的亚细胞定位信息。本文通过综述Bi FC技术的原理、优缺点及其在ND研究领域中的应用进展,探讨该技术对揭示寡聚体和包涵体形成原因方面的潜力,为神经系统疾病开发新的治疗策略提供重要参考。
The pathological feature of degenerative diseases of the nervous system (ND) is the neurotoxicity that proteins accumulate in or extracellularly as an accumulation of deposits. Bi-Fluorescent Complementation (Bi FC) is an important tool for studying the molecular mechanism of ND-related protein oligomerization. Unlike other technologies, Bi FC not only visualizes protein-protein interactions in the physiological environment but also provides detailed subcellular location information. In this paper, by reviewing the principles, advantages and disadvantages of BiFC technology and its applications in the field of ND research, this article explores the potential of this technology to reveal the reasons for the formation of oligomers and inclusion bodies and provides important new strategies for the development of new therapeutic strategies for neurological diseases reference.