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将商品化 Profiler Plus和 Cofiler扩增检测试剂盒用于亲子鉴定案例和无关个体血样的分析。 186例亲子鉴定案中，母－子－假设父三联体 149例，假设父－子二联体 37例。在 149例含双亲和孩子的鉴定中，有 127例认定存在亲生血缘关系，其 RCP值平均为 99.98％；其余 22例排除了被检父为孩子生父，排除机率为 14.77％，排除指标平均为 6.1个。在 37例含单亲及孩子的鉴定中，有 23例认定存在亲生血缘关系，其 RCP值平均为 99.92％；其余 14例排除被检父为孩子生父，排除机率为 37.84％，平均有 5个不吻合的 STR位点出现。若特意省去三人组合案例中的母亲，将其作为单亲案件进行统计，仅检测 Profiler Plus体系的 9个 STR位点时，则 RCP值平均为 99.1959％，案例中 21.51％将不能得出明确的结论。同时检测 Profiler Plus和 Cofiler体系共 13个 STR位点，则案例中 97.31％能排除或认定生父，案例中 2.69％通过补充母亲样本能排除或认定生父。 Cofiler体系中 6个 STR位点的累积排除率达 0.9825。上述数据表明， Cofiler试剂盒在单亲鉴定案件中有着重要的应用价值。
The commercial Profiler Plus and Cofiler Amplification Assay kits were used for paternity testing and analysis of unrelated individual blood samples. 186 cases of paternity test, the mother - son - hypothetical triplet 149 cases, assuming the father - son duplex 37 cases. Of the 149 cases with both parents and children, 127 were identified as having a natural blood relationship, with an average RCP value of 99.98%. The remaining 22 cases excluded the father who was the father of the child, with an exclusion rate of 14.77% and an average of 6.1. Among the 37 cases with single parents and children, 23 cases were found to have a natural blood relationship with an average RCP value of 99.92%. The remaining 14 cases excluded the father as the child’s father, with an exclusion rate of 37.84% and an average of 5 cases Anastomosed STR sites appear. If the mothers in the triad case were deliberately omitted and counted as single-parent cases, only the RCP value was 99.1959% when only the 9 STR loci of the Profiler Plus system were tested, and 21.51% of the cases would not be able to draw a clear Conclusion. While detecting a total of 13 STR loci in the Profiler Plus and Cofiler systems, 97.31% of the cases could exclude or identify the biological father, and 2.69% of the cases could exclude or identify the biological father by supplementing the mother’s sample. The cumulative exclusion rate of 6 STR loci in Cofiler system was 0.9825. The above data show that Cofiler kit has important application value in single-parent identification cases.