论文部分内容阅读
目的:采用高浓度胰岛素体外诱导培养HepG2细胞,建立胰岛素抵抗的细胞模型,观察齐墩果酸对HepG2细胞胰岛素抵抗的影响。方法:用CCK-8法筛选齐墩果酸作用于HepG2细胞的实验浓度,然后用高浓度胰岛素诱导HepG2细胞胰岛素抵抗,给予不同浓度的齐墩果酸(0.1、1、10、100μmol·L-1)、吡格列酮(10μmol·L-1)干预,以葡萄糖氧化酶法检测葡萄糖消耗量。结果:1不同浓度齐墩果酸对HepG2细胞增殖无明显影响。2与对照组比较,高浓度胰岛素作用24 h后,模型组葡萄糖消耗量明显降低(P<0.01);与模型组比较,齐墩果酸组HepG2细胞葡萄糖消耗量随浓度增加而增加,成一定的量效关系,浓度为10μmol·L-1和100μmol·L-1时葡萄糖消耗量显著增加(P<0.01或P<0.05)。结论:齐墩果酸能改善高浓度胰岛素诱导的HepG2细胞胰岛素抵抗。
OBJECTIVE: To study the effect of oleanolic acid on the insulin resistance of HepG2 cells induced by high concentration of insulin induced HepG2 cells in vitro. Methods: The experimental concentration of oleanolic acid (HepG2) cells was screened by CCK-8 method, then the insulin resistance of HepG2 cells was induced by high concentration of insulin, and different concentrations of oleanolic acid (0.1,1,10,100μmol·L- 1), and pioglitazone (10μmol·L-1). Glucose oxidase was used to detect glucose consumption. Results: 1 Oleanolic acid had no significant effect on the proliferation of HepG2 cells. Compared with the control group, the glucose consumption of model group decreased significantly (P <0.01) 24 h after high-concentration insulin treatment. Compared with the model group, the glucose consumption of HepG2 cells in the oleanolic acid group increased with the increase of concentration, (P <0.01 or P <0.05). At the concentrations of 10 μmol·L-1 and 100 μmol·L-1, the glucose consumption increased significantly (P <0.01 or P <0.05). Conclusion: Oleanolic acid can improve insulin resistance induced by high concentration of insulin in HepG2 cells.