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目的用扫描电镜对巴氏染色正常形态≤4%的畸形精子增多症患者的精子微细结构进行观察分析。方法经改良巴氏染色后正常形态≤4%定为实验组,正常形态>14%定为对照组。两组患者的精液标本在扫描电镜下观察,并将结果进行分析比较。结果精子顶体表面光滑率对照组88.21%,实验组45.34%;顶体缺失率对照组0.30%,实验组3.00%;环状沟平滑对照组72.56%,实验组47.68%;环状沟呈波浪状对照组13.12%,实验组37.32%;线粒体鞘异常对照组12.75%,实验组53.22%;胞浆小滴对照组0.90%,实验组2.60%;尾部畸形对照组6.74%,实验组17.46%,两组比较差异均有显著性(P<0.05)。结论正常形态≤4%的畸形精子增多症患者精子除宏观结构有异常外,顶体、环状沟、线粒体、尾部等微细结构异常发生率增加。
Objective To observe and analyze the fine structure of sperm in patients with abnormal spermatogenesis by normal scanning electron microscopy (SEM). Methods The normal morphology of the modified Papanicol staining ≤ 4% as the experimental group, the normal morphology> 14% as the control group. The semen specimens of two groups were observed under scanning electron microscope and the results were analyzed and compared. Results The sperm acrosome surface smoothness was 88.21% in the control group, 45.34% in the experimental group, 0.30% in the control group and 3.00% in the experimental group, 72.56% in the annular groove smooth control group and 47.68% in the experimental group, 13.12% in the control group, 37.32% in the experimental group, 12.75% in the mitochondrial sheath abnormality control group, 53.22% in the experimental group, 0.90% in the cytoplasmic droplet control group, 2.60% in the experimental group, 6.74% in the tail malformation control group and 17.46% in the experimental group, The differences between the two groups were significant (P <0.05). Conclusions In addition to the abnormal macrostructures, sperm, ring ditch, mitochondria, tail and other microstructural abnormalities in patients with abnormal spermatogenesis with normal morphology ≤4% increase.