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利用RAPD分析技术,选取22个20个碱基长度的随机引物,对7种野生菊花、14种栽培菊花和5个种间杂种进行了随机扩增。通过实验建立了PCR随机扩增实验体系。在观察到的224个扩增条带中,34条(15%)表现多态性。利用UPGMA法对扩增数据进行分析,结果表明:在7种野生种中,Dendranthemaindicum、D.vestitum和D.nankingense与栽培菊花亲缘关系最近,而D.zawadski与栽培菊花亲缘关系最远。前3种野菊与栽培菊花间的遗传距离小于0.40,而D.zawadski与栽培菊花间的遗传距离大于0.50。根据上述数据及以往研究结果,使用RAPD数据对菊花起源问题进行了探讨,提出栽培菊花主要起源于D.indicum、D.vestitum和D.nankingense.
Using RAPD analysis, 22 random primers of 20 bases length were selected and randomly amplified from 7 wild chrysanthemums, 14 cultivated chrysanthemums and 5 interspecific hybrids. The experimental random amplified PCR experiment system was established. Of the 224 amplified bands observed, 34 (15%) showed polymorphisms. Using UPGMA analysis of amplification data, the results showed that: in seven wild species, Dendranthe maindicum, D. vestitum and D. nankingense and cultivated chrysanthemum closest, and D. Zawadski and cultivation of chrysanthemum furthest. The genetic distance between the first 3 wild chrysanthemums and cultivated chrysanthemum is less than 0.40, while D. The genetic distance between zawadski and cultivated chrysanthemum is greater than 0.50. According to the above data and previous research results, the origin of chrysanthemum was discussed using RAPD data. indicum, D. vestitum and D. nankingense