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BK病毒(BKV)DNA籍钙沉淀技术感染293细胞.感染细胞(称作293-BK细胞)如其他293细胞一样生长,14天后可在软琼脂中形成细胞集落.293-BK细胞类似293细胞,为一种大的上皮样细胞,有时细胞核稍肿胀.它们生长致密后还继续分裂,形成小岛或团块;在液体培养中形成大的细胞聚集物.293-BK细胞DNA与标记的BKV DNA或互补RNA的杂交试验提示,病毒基因组或BKV DNA顺序存在于293-BK细胞中.这也被细胞DNA与标记病毒DNA原位杂交和DNA-DNA复性动力学试验所证实.检查了293-BK细胞培养物的血凝作用,结果显示293-BK细胞不发放BKV.
293 cells were infected with BKV DNA by calcium precipitation technique.The infected cells (called 293-BK cells) grew like other 293 cells and colonies formed in soft agar after 14 days.293-BK cells resemble 293 cells, Is a large epithelial-like cell, sometimes with slightly swollen nuclei, which continue to divide after it has grown densely to form islets or lumps and form large aggregates of cells in liquid culture.229 The DNA of the BK cell binds to the labeled BKV DNA Or hybridization with complementary RNAs suggests that the viral genome or BKV DNA sequence is present in 293-BK cells as evidenced by in situ hybridization of DNA with labeled viral DNA and DNA-DNA refolding kinetics. The 293- BK cell cultures showed no BKV release from 293-BK cells.