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目的观察蛇床子素对小鼠高脂性脂肪肝的治疗作用。方法通过给小鼠灌服脂肪乳4周建立小鼠高脂性脂肪肝模型。将已形成脂肪肝的小鼠随机分为蛇床子素10 mg/kg、20 mg/kg、40 mg/kg组和脂肪肝模型组,每组小鼠10只,连续灌胃给药6周后,检测血清和肝组织中总胆固醇(TC)、甘油三酯(TG)、游离脂肪酸(FFA)和肝质量系数等指标,并用光镜检查肝组织的形态学改变。结果高脂性脂肪肝小鼠经蛇床子素10～40 mg/kg治疗6周后,血清和肝组织中TC、TG、FFA和肝质量系数明显降低(P<0.05或P<0.01),并呈一定的剂量依赖趋势。光镜检查结果显示,给予蛇床子素治疗的小鼠肝细胞脂肪变性的程度明显减轻。结论蛇床子素对小鼠高脂性脂肪肝具有一定的治疗作用。
Objective To observe the therapeutic effect of osthole on fatty liver in mice. Methods Mouse model of hyperlipidemic fatty liver was established by intramuscular injection of fat milk for 4 weeks. The mice with fatty liver were randomly divided into osthole 10 mg / kg, 20 mg / kg, 40 mg / kg group and fatty liver model group, 10 mice in each group, after continuous gavage for 6 weeks The levels of total cholesterol (TC), triglyceride (TG), free fatty acid (FFA) and liver mass index in serum and liver tissue were detected. The morphological changes of liver tissue were examined by light microscopy. Results The TC, TG, FFA and liver mass index of serum and liver tissues were significantly decreased (P <0.05 or P <0.01) in the hyperlipidemic fatty liver mice treated with osthole 10 ~ 40 mg / kg for 6 weeks Certain dose-dependent trends. Light microscopic examination showed that the degree of steatosis of hepatocytes in mice treated with osthole was significantly reduced. Conclusion Osthole on mice with fatty liver fatty liver has a certain therapeutic effect.