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为测定动物组织中对预防动脉粥样硬化有效的成分-牛磺酸的含量．利用渗透压原理，以水为溶剂先提取动物组织中牛磺酸，用真空冷冻抽干浓缩后进行柱前衍生处理、进样．经反相高效液相色谱柱内疏水分离后，再用标准曲线法进行定量测定。在选定的试验条件下，牛磺酸保留时间为5．06min，分离效果好，峰形呈正态分布，样品溶液相对标准偏差为1．5％．最低检出量是4.48ng，牛磺酸含量在5．58X103～1．79X105μg／L范围，色谱峰面积与牛磺酸含量间有良好线性关系（r=0．9998），131．2～9682ng牛磺酸的样品加标回收率为97．79／～101．99％。按照此法测得蛞蝓组织中牛磺酸含量为75．4μg／g。此方法简便、准确，抗干扰能力强，是一种测定动物中微量牛磺酸的比较完善的分析方法。
To determine the composition of animal tissue to prevent atherosclerosis - taurine content. The principle of osmotic pressure was used to extract the taurine from animal tissues with water as the solvent, and the mixture was vacuum-freeze-dried and concentrated before being subjected to pre-column derivatization. After reversed-phase high performance liquid chromatography column hydrophobic separation, and then the standard curve method for quantitative determination. Under the selected experimental conditions, the retention time of taurine is 5.06min, the separation effect is good, the peak shape is normal distribution, the relative standard deviation of the sample solution is 1.5%. The minimum detectable amount was 4.48ng, the taurine content was in the range of 5.58X103 ~ 1.79X105μg / L, and there was a good linear relationship between chromatographic peak area and taurine content (r = 0.9998), 131.2-9682ng Taurine sample spike recovery was 97.79 / ~ 101.99%. According to this method measured taiga tissue taurine content of 75.4μg / g. This method is simple, accurate, anti-interference ability, is a kind of determination of trace amounts of animal taurine analysis method.