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用ＤｅｘｔｒａｎＴ５００，ＰＥＧ３３５０两相体系制备玉米根质膜．首先在高盐浓度（２２ｍｍｏｌ／ＬＮａＣｌ）下选用五种不同的聚合物浓度（５．８％、６．０％、６．２％、６．３％、６．４％，Ｗ／Ｗ），研究了玉米根质膜在两相体系中的分配情况，在此基础上进一步研究了Ｎａ－Ｃｌ浓度（２、４、５、１１、２２ｍｍｏｌ／Ｌ）对玉米根质膜的纯度及得率的影响．结果表明，制备玉米根质膜选用６．２％（Ｗ／Ｗ）聚合物浓度，７．５ｍｍｏｌ／ＬＮａＣｌ的两相体系比较合适．标志酶鉴定及低ｐＨ值磷钨酸染色电镜检测均表明获得了高纯度密实的正向型的质膜囊泡，质膜标志酶ＶＯ３－４－ＡＴＰａｓｅ的活性潜势达８８．９％．
Corn root plasma membrane was prepared with DextranT500 and PEG3350 two-phase system. Five different polymer concentrations (5.8%, 6.0%, 6.2%, 6.3%, 6.4%, W / W) were first selected at high salt concentrations (22 mmol / L NaCl) The distribution of maize root plasma membrane in two-phase system was studied. On the basis of this, the purity and yield of corn root plasma membrane were further studied by Na-Cl concentration (2,4,5,11,22mmol / L) influences. The results showed that a 6.2% (W / W) polymer concentration and a 7.5mmol / L NaCl two-phase system were suitable for the preparation of corn root plasma membrane. Marker enzyme identification and low pH value tungstophosphoric acid staining electron microscopy showed that a high purity dense positive membrane vesicles were obtained, and the activity potential of plasma membrane marker enzyme VO3-4-ATPase reached 88.9%.