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目的 探索最佳的豆腐法炮制草乌的工艺参数.方法 采用L16(43)正交试验方法,以浸漂时间,水煮时间和豆腐用量3因素,各设置4水平进行16次试验,每次实验结果均以单酯型和双酯型生物碱总量测定数据为评判指标.结果 浸漂时间4水平4次试验结果的单酯型生物碱含量分别为0.11％,0.07％,0.062％和0.048％;水煮时间4次结果的含量分别为0.081％,0.066％,0.074％和0.067％;豆腐用量4次结果的含量分别为0.070％,0.072％,0.080％和0.073％;双酯型生物碱16次测定结果均＜0.034％.3因素中对单酯型生物碱含量影响大小的次序为浸漂时间＞水煮时间＞豆腐用量.在实验条件下,毒性成分双酯型生物碱均小于中国药典的限度要求,符合规定,而毒性成分双酯型生物碱总量随豆腐用量的增加而明显减少.结论 豆腐法炮制草乌的最佳工艺条件为A3B3C2,即浸漂6d,水煮5h,加豆腐25％.“,”OBJECTIVE To explore the best parameters of processing Aconiti Kusnezoffii Radix by bean curd.METHODS Orthogonal test [L16(43)] was adopted to investigate soaking time,decocting time and bean curd proportion on the processing technology,in which sixteen experiments were set up.The monoester alkaloids and diester-alkaloids were measured as the evaluation indexes.RESULTS The contents of monoester alkaloids were 0.11％,0.07％,0.062％ and 0.048％ respectively under the four levels of soaking time;0.081％,0.066％,0.074％,0.067％ respectively under the four levels of decocting time;0.070％,0.072％,0.080％,0.073％ respectively under the four levels of bean curd proportion.The contents of diester-alkaloids of the 16 processed samples were all ＜0.034％.The order of the influence on the content of monoester alkaloids was:soaking time＞decocting time ＞ bean curd proportion.Under any of the above conditions,the diester-alkaloids were all less than the limit of Chinese Pharmacopoeia.The contents of diester-alkaloids were reduced clearly with the increase of the amount of bean curd.CONCLUSION The best parameters of the processing are as follows:soaking time is 5 d,decocting time is 5 h and bean curd proportion is 25％ of medicinal herbs.