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目的探讨氢醌对人淋巴母细胞TK6细胞生长及凋亡的影响。方法 1采用9×4析因实验设计方法,以浓度为0.0、2.5、5.0、10.0、20.0、40.0、80.0、160.0、320.0μmol/L氢醌分别处理TK6细胞6.0、12.0、24.0和48.0 h,以磷酸盐缓冲液(PBS)作为氢醌溶剂。采用细胞计数试剂盒(CCK-8)检测细胞存活率以了解氢醌对TK6细胞增殖抑制能力。2根据CCK-8分析结果,选择后续实验的氢醌处理浓度和处理时间,采用磷脂结合蛋白V/碘化丙啶流式细胞分析法检测细胞凋亡情况,化学发光法检测细胞内天冬氨酸特异性半胱氨酸蛋白酶(Caspase)3/7活力。结果 1氢醌处理浓度和处理时间存在交互效应(P<0.01);以浓度为2.5、5.0、10.0、20.0和40.0μmol/L的氢醌处理TK6细胞6.0、12.0和24.0 h时,绝大多数细胞存活率出现升高情况,以浓度为80.0、160.0和320.0μmol/L的氢醌处理TK6细胞6.0、12.0、24.0和48.0 h时,细胞存活率均出现下降情况。最终选择0.0~40.0μmol/L的氢醌处理TK6细胞24.0 h作为后续实验方案。2当处理浓度为0.0~40.0μmol/L时,氢醌诱导的TK6细胞凋亡随着处理浓度的增加而增加,呈剂量-效应关系,回归方程为^y=0.891 x+5.478[决定系数(R2)=0.981,F=831.33,P<0.01];氢醌诱导的TK6细胞Caspase 3/7活力随着处理浓度的增加而增加,呈剂量-效应关系,回归方程为^y=0.201 x+2.200(R2=0.932,F=219.77,P<0.01)。结论氢醌能抑制TK6细胞生长并可诱导其凋亡,其过程与细胞内Caspases 3和Caspase 7途径有关。
Objective To investigate the effect of hydroquinone on the growth and apoptosis of human lymphoblasts TK6 cells. Method 1 The 9 × 4 factorial design method was used to treat TK6 cells at the concentrations of 6.0, 2.0, 24.0 and 48.0 h respectively at the concentrations of 0.0, 2.5, 5.0, 10.0, 20.0, 40.0, 80.0, 160.0 and 320.0 μmol / Phosphate buffer (PBS) was used as hydroquinone solvent. Cell viability was measured by using cell counting kit (CCK-8) to understand the inhibitory effect of hydroquinone on TK6 cell proliferation. 2 According to the results of CCK-8 analysis, the concentration of hydroquinone and the treatment time were selected in the follow-up experiments. The apoptosis of cells was detected by flow cytometry with phospholipid binding protein V / propidium iodide and the chemiluminescence method was used to detect the intracellular aspartate Acid specific cysteine protease (Caspase) 3/7 activity. Results 1 The interaction between hydroquinone concentration and treatment time was significant (P <0.01). When TK6 cells were treated with 2.5, 5.0, 10.0, 20.0 and 40.0 μmol / L hydroquinone for 6.0,12.0 and 24.0 h, The survival rate of cells increased. The TK6 cells treated with hydroquinone at concentrations of 80.0, 160.0 and 320.0 μmol / L for 6.0,12.0,24.0 and 48.0 h all showed a decrease in cell viability. Finally, 0.0 ~ 40.0μmol / L hydroquinone was used to treat TK6 cells for 24.0 h as the follow-up experimental protocol. 2 When treated with 0.0 ~ 40.0μmol / L, the apoptosis of TK6 cells induced by hydroquinone increased with the increase of the concentration, and the dose-response relationship was shown. The regression equation was y = 0.891x + 5.478 [ R2 = 0.981, F = 831.33, P <0.01]. Caspase 3/7 activity of TK6 cells induced by hydroquinone increased with the increase of dose, and the regression equation was y = 0.201 x + 2.200 (R2 = 0.932, F = 219.77, P <0.01). Conclusion Hydroquinone can inhibit the growth of TK6 cells and induce its apoptosis. The process is related to the intracellular Caspases 3 and Caspase 7 pathways.