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目的:采用HPLC同时测定荷叶饮片中4种黄酮类成分的含量,为制定荷叶饮片的质量标准提供实验依据。方法:采用Kromasil C18(4.6 mm×200 mm,5μm)色谱柱,以甲醇(A)-0.2%磷酸水(B)为流动相,梯度洗脱(0~8 min,10%~40%A;8~23 min,40%~70%A;23~30 min,70%~85%A),流速1 mL·min-1,柱温30℃,二极管阵列检测器,检测波长360 nm。结果:槲皮素-3-O-桑布双糖苷、金丝桃苷、异槲皮苷和槲皮素的线性范围依次为6.10~97.60,18.76~300.16,8.48~135.68,9.12~145.92 mg·L-1,平均回收率(n=6)依次为100.98%(RSD 1.85%),99.05%(RSD 1.48%),99.29%(RSD 1.84%),97.71%(RSD 1.78%)。5个不同产地荷叶饮片中槲皮素-3-O-桑布双糖苷、金丝桃苷、异槲皮苷和槲皮素的含量分别为0.092%~0.161%,0.949%~1.862%,0.067%~0.133%,0.040%~0.062%。结论:该方法简便快速,准确可靠,可作为荷叶饮片中4种黄酮成分的含量测定方法。不同产地荷叶饮片中上述4种黄酮成分含量相差较大,建议将黄酮成分纳入荷叶饮片的质量控制标准。
OBJECTIVE: To determine the contents of four flavonoids in lotus leaf slices by HPLC simultaneously, and to provide experimental evidence for establishing the quality standard of lotus leaf slices. Methods: Kromasil C18 (4.6 mm × 200 mm, 5 μm) column was used to elute gradient (0-8 min, 10% -40% A) with methanol (A) 8 to 23 min, 40% to 70% A, 23 to 30 min, 70% to 85% A), flow rate 1 mL · min-1 and column temperature 30 ℃. Results: The linear ranges of quercetin-3-O-bambuteride, hyperin, quercetin and quercetin were 6.10 ~ 97.60, 18.76 ~ 300.16, 8.48 ~ 135.68 and 9.12 ~ 145.92 mg · L-1. The average recoveries (n = 6) were 100.98% (RSD 1.85%), 99.05% (RSD 1.48%), 99.29% (RSD 1.84%) and 97.71% (RSD 1.78%), respectively. The contents of quercetin-3-O-, mulberobioside, hyperoside and quercetin in lotus leaf decoction in five different areas were 0.092% -0 0.161%, 0.949% -1.862% 0.067% ~ 0.133%, 0.040% ~ 0.062%. Conclusion: The method is simple, rapid, accurate and reliable and can be used as a method for the determination of four flavonoids in lotus leaf decoction. Different origin lotus leaf Pieces of the four flavonoids content difference between the larger, it is proposed to incorporate flavonoids into lotus leaf quality control standards.