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目的探索波长为635 nm的激光对新西兰兔眼晶状体的氧化损伤效应。方法 1在体激光暴露实验。健康雌性新西兰兔5只,随机取2只兔为空白对照组(4只眼),另3只兔的右眼为暴露组(3只眼),左眼为自身对照组(3只眼);暴露组兔眼以照射功率为20 m W的635 nm激光连续照射2 h/d,每周6 d,共60 d,2个对照组兔眼均不进行激光暴露;实验结束后分离各组晶状体进行透明度检查,采用酶标仪测定晶状体超氧化物歧化酶(SOD)活力和丙二醛(MDA)、水溶性蛋白(WSF)、脲溶性蛋白(USF)的水平,计算WSF占总蛋白百分比(%)。2离体激光暴露实验。健康雌性新西兰兔4只,分离晶状体置于无菌M199培养基孵育后,以左眼为自身对照组(4只眼),右眼为暴露组(4只眼);暴露组晶状体以照射功率为20 m W的635 nm激光照射8 h/d,连续照射5 d;自身对照组晶状体不进行激光暴露。采用酶标仪测定晶状体SOD活力和MDA水平。结果在体激光暴露实验中,暴露组1只晶状体后囊部位出现轻微混浊,Geraldin分级为1级,其余各组晶状体均未发现浑浊现象,Geraldin分级为0级;暴露组、自身对照组和空白对照组3组晶状体的SOD活力和MDA水平分别比较,差异均无统计学意义[SOD:(1.803±0.542)vs(1.982±0.513)vs(1.247±0.235)mmol/(min·gprot),P>0.05;MDA:(0.078±0.017)vs(0.080±0.005)vs(0.077±0.006)μmol/gprot,P>0.05];3组晶状体WSF、USF的水平和WSF占总蛋白百分比分别比较,差异均无统计学意义[WSF:(113.31±14.70)vs(118.75±12.91)vs(114.05±14.41)g/L,P>0.05;USF:(3.23±0.22)vs(3.04±0.48)vs(2.52±0.50)g/L,P>0.05;WSF占总蛋白百分比:(97.33±0.30)%vs(97.39±0.25)%vs(97.79±0.70)%,P>0.05]。离体激光暴露实验中,暴露组晶状体SOD活力低于自身对照组[(0.144±0.004)vs(0.172±0.004)mmol/(min·gprot),P<0.05];2组晶状体MDA水平比较,差异无统计学意义[(0.031±0.002)vs(0.030±0.003)μmol/gprot,P>0.05]。结论 635 nm激光暴露可能会导致晶状体氧化损伤反应,具体机制有待进一步的研究。
Objective To explore the oxidative damage effects of 635 nm laser on the lens of New Zealand rabbits. Method 1 In vivo laser exposure experiments. Five healthy New Zealand white rabbits were randomly divided into two groups: the control group (4 eyes), the other 3 rabbits (3 eyes) and the left eye as the control group (3 eyes). The rabbits in the exposed group were irradiated with 635 nm laser light with a power of 20 mW for 2 h / d for 6 days per week for 60 days. No laser exposure was observed in the two control eyes. After the experiment, The transparency of the lens was measured. The SOD activity and the content of malondialdehyde (MDA), water-soluble protein (WSF) and urea-soluble protein (USF) %). 2 in vitro laser exposure experiments. Four healthy female New Zealand rabbits were divided into four groups. The isolated lens was placed in sterile M199 medium, the left eye was the control group (4 eyes) and the right eye was exposed group (4 eyes). The exposure group 20 mW 635 nm laser irradiation 8 h / d, continuous irradiation 5 d; self-control group, the lens is not exposed to laser. The activity of SOD and the level of MDA in lens were measured by microplate reader. Results In the body laser exposure experiment, Geraldin was classified as grade 1 in the posterior capsule of the exposed group, and no turbidity was observed in the other groups. Geraldin grade 0 was graded. The exposure group, the self-control group and the blank There was no significant difference in SOD activity and MDA level between the three groups of the control group (SOD: 1.803 ± 0.542 vs 1.982 ± 0.513 vs 1.247 ± 0.235 mmol / min / gprot, P> 0.05; MDA: (0.078 ± 0.017) vs (0.080 ± 0.005) vs (0.077 ± 0.006) μmol / gprot, P> 0.05]. There was no difference in WSF, USF and WSF P <0.05); USF: (3.23 ± 0.22) vs (3.04 ± 0.48) vs (2.52 ± 0.50) vs g / L, P> 0.05; WSF percentage of total protein: (97.33 ± 0.30)% vs (97.39 ± 0.25)% vs (97.79 ± 0.70)%, P> 0.05]. In the ex vivo laser exposure experiment, the SOD activity of the exposed lens group was lower than that of the self-control group [(0.144 ± 0.004) vs (0.172 ± 0.004) mmol / (min · gprot), P <0.05] No statistical significance [(0.031 ± 0.002) vs (0.030 ± 0.003) μmol / gprot, P> 0.05]. Conclusion 635 nm laser exposure may lead to oxidative damage of lens. The exact mechanism remains to be further studied.