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目的:观察姜半夏乙醇提取物对人胃癌SGC7901细胞增殖和凋亡的影响。方法:不同浓度姜半夏乙醇提取物(终浓度为1mg/ml,0.5mg/ml,0.25mg/ml,0.125mg/ml)处理SGC7901细胞后,倒置相差显微镜下观察细胞的形态学变化;通过甲基噻唑基四唑法检测细胞增殖状况、描绘生长曲线,使用紫外分光光度法观察药物干预后细胞ATP酶活力;AnnexinV-异硫氰酸荧光素(fluorescein isothiocyanate,FITC)/碘化丙啶(propidium iodide,PI)双标记法流式细胞术检测姜半夏乙醇提取物对SGC7901细胞诱导凋亡的情况。结果:不同浓度姜半夏乙醇提取物均能不同程度地抑制人胃癌SGC7901细胞的增殖;在姜半夏乙醇提取物诱导细胞后细胞发生了边缘毛刺、体积缩小等形态学变化,同时可见细胞折光度和贴壁能力下降;AnnexinV-FITC/PI双标记法检测显示姜半夏乙醇提取物可诱导细胞发生凋亡;细胞总ATP酶活力在药物干预72小时后出现明显下降;并且随着药物浓度增加细胞凋亡率、细胞形态异常改变以及ATP酶活力抑制作用均呈上升趋势。结论:姜半夏乙醇提取物可抑制人胃癌SGC7901细胞的增殖,促进其凋亡,抑制细胞ATP酶活力。
Objective: To observe the effect of ethanol extract of ginger, Pinellia ternata on the proliferation and apoptosis of human gastric cancer cell line SGC7901. Methods: SGC7901 cells were treated with different concentrations of ethanol extract of Ginger Pinellia (the final concentrations were 1mg / ml, 0.5mg / ml, 0.25mg / ml and 0.125mg / ml). The morphological changes of cells were observed under inverted phase contrast microscope. Thiazolyl tetrazolium assay was used to detect the proliferation of cells and the growth curve was drawn. The activity of ATPase was observed by UV spectrophotometry. AnnexinV fluorescein isothiocyanate (FITC) / propidium iodide , PI) double labeling method flow cytometry detection of ethanol extract of gingerbai pinellia SGC7901 cells induced apoptosis. Results: The ethanol extract of Ginger Pinellia could all inhibit the proliferation of human gastric cancer SGC7901 cells to varying degrees. Morphological changes such as edge burr and volume reduction were observed in the cells induced by ethanol extract of Ginger Pinellia, and at the same time, the cell refraction and adhesion Ability to reduce; AnnexinV-FITC / PI double-labeled assay showed that ethanol extract of ginger and Pinellia can induce apoptosis of cells; total ATPase activity in the drug intervention after 72 hours significantly decreased; and with the drug concentration increased apoptosis rate, Cell morphology changes and ATPase activity inhibition showed an upward trend. Conclusion: Jiangxiaxia ethanol extract can inhibit the proliferation of human gastric cancer SGC7901 cells, promote apoptosis and inhibit the activity of ATPase.