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几年来，着重从接种材料类型、花粉发育时期、低温预处理、去分化和分化培养基及其附加成分以及它们对花粉植株秧苗素质的影响等方面进行了系统的研究。进一步明确了北方粳稻花药培养技术的几个关键性环节，使粳稻花药培养效率大大提高。研究结果表明：各种类型的粳稻接种后都能成功地诱导出愈伤组织和长成植株。但杂合程度高的材料诱导频率明显高于纯会品种；接种花药小施子发育时期以单核中、晚期诱导效果最好；整株取下进行5～7℃低温预处理8～16天，诱导率最高可达284％；去分化培养基以N6＋24－D2mg／l＋蔗糖5％＋琼脂粉0．6％，pH值5．8；分化培养基以MS＋BA2mg／l＋IAA0．2～0．5mg／l＋CH500～800mg／l＋蔗糖3％＋琼脂粉06％，pH值5．8；壮苗培养基以1／2MS大量＋NAA0．1～0．2mg／l＋MET2mg／l＋蔗糖2％＋琼脂粉0．6％，pH值5．8为最好。将壮苗培养基中的MET提高到4mg／l，在15℃、弱光条件下保存，可使花粉植株安全越冬，于翌年5月中旬移栽大田，发育正常。
In recent years, systematic studies have focused on the types of inoculation material, pollen development period, low temperature pretreatment, dedifferentiation and differentiation of media and their additional components, and their impact on the quality of pollen plant seedlings. Further clarified several key aspects of northern japonica anther culture technology, so that the efficiency of Japonica anther culture greatly increased. The results showed that all kinds of japonica rice could successfully induce callus and grow into plants after inoculation. However, the induction frequency of materials with high degree of heterozygosity was significantly higher than that of pure varieties. The monolayer inoculation anthers showed the best induction effect in the late stage and the whole plant was removed and pretreated with low temperature for 5 ~ 7 ℃ for 8 ~ 16 days , The induction rate was up to 284%; differentiation medium with N6 + 24-D2mg / l + sucrose 5% + agar powder 0.6%, pH 5.8; differentiation medium with MS + BA2mg / l + IAA0.2 ~ 0.5mg / l + CH500 ~ 800mg / l + sucrose 3% + agar powder 06%, pH 5.8; strong seedling medium to 1 / 2MS large amount of + NAA0.1 ~ 0.2mg / l + MET2mg / l + sucrose 2% agar powder 0.6% , PH 5.8 is the best. Increasing the MET concentration in the seedling growth medium to 4mg / l and preserving the pollen plants under 15 ℃ and low light conditions allowed pollen plants to overwinter safely and transplanted into the field in mid-May of the next year with normal development.