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采用荧光光谱研究了电子受体氯冉酸与电子给体吡哌酸之间的荷移反应。实验结果表明,在丙酮-甲醇介质中即可生成稳定的n-π络合物,其荧光发射显著增强。据此提出了一种基于荷移反应简便可靠地测定吡哌酸的荧光光谱新方法,用曲线交叉法和平衡移动法测定络合物的组成比为1∶1。线性范围为0.10~7.0μg/mL,最低检出限为0.10μg/mL,回收率为97.4%~99.3%,相对标准偏差为1.3%~2.6%。最后探讨了荷移反应的机理,该方法用于PIP药片的测定,结果令人满意。
Fluorescence spectroscopy was used to study the charge-transfer reaction between the electron acceptor choronic acid and the electron donor pipemidic acid. The experimental results show that the stable n-π complex can be formed in acetone-methanol medium, and the fluorescence emission is significantly enhanced. Therefore, a new fluorescence spectrometry method for the determination of pipemidic acid based on the charge-transfer reaction was proposed. The composition ratio of the complex was determined to be 1: 1 by curve crossing method and equilibrium shift method. The linear range was 0.10-7.0μg / mL. The detection limit was 0.10μg / mL. The recoveries were 97.4% -99.3%. The relative standard deviations were 1.3% -2.6%. Finally, the mechanism of charge transfer reaction was discussed. The method was applied to the determination of PIP tablets with satisfactory results.