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目的:评价流式细胞术与补体依赖性微量淋巴细胞毒试验检测强直性脊柱炎(AS)患者HLA-B27。方法:采用美国Beckman-Coulter公司的EpicsXL流式细胞仪及配套试剂与补体依赖性微量淋巴细胞毒性试验对已诊断为强直性脊柱炎患者124例进行检测,以验证检测的准确性。结果:用流式细胞术检测124例AS患者,阳性率为91.1%,用补体依赖性微量淋巴细胞毒试验检测124例AS患者,阳性率为34.9%。两个方法学实验间比较差异有统计学意义(P<0.001)。结论:用流式细胞术检测结果稳定,阳性率高,能早期对AS患者的诊断,而补体依赖性微量淋巴细胞毒性试验结果不稳定,阳性率低,容易造成误诊。
Objective: To evaluate flow cytometry and complement-dependent microlymphocytotoxicity assay for detection of HLA-B27 in patients with ankylosing spondylitis (AS). Methods: 124 patients diagnosed as ankylosing spondylitis were tested with EpicsXL flow cytometer and complementary reagents and complement-dependent microlymphocytotoxicity test from Beckman-Coulter Company in USA to verify the accuracy of the test. Results: 124 cases of AS patients were detected by flow cytometry, the positive rate was 91.1%. The complement-dependent microlymphocytotoxicity test was used to detect 124 AS patients, the positive rate was 34.9%. There was a significant difference between the two methodological experiments (P <0.001). Conclusion: The results of flow cytometry were stable and the positive rate was high. Early diagnosis of AS patients was possible. The results of complement-dependent microlymphocytotoxicity test were unstable and the positive rate was low, which could lead to misdiagnosis.