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目的检测涎腺黏液表皮样癌(mucoepidermoid carcinoma,MEC)、正常涎腺组织(normal salivary gland,NSG)中Cox-2、Ki-67蛋白的表达情况,为MEC预后评估提供可筛选的标志物。方法应用免疫组化染色方法(PV-6001二步法)检测11例NSG、34例MEC中Cox-2、Ki-67的表达,采用SPSS 16.0软件对结果进行统计学分析。结果 MEC、NSG中Cox-2的表达差异有统计学意义(P<0.01);在不同病理类型(高、中、低分化)、是否复发、是否转移MEC中Cox-2的表达差异有统计学意义(P<0.05,P<0.01),而在不同性别、年龄、民族、部位比较差异无统计学意义。Ki-67在MEC与NSG中表达差异有统计学意义(P<0.01),在高、中与低分化间、不同部位、是否复发、是否转移中的表达差异有统计学意义(P<0.05,P<0.01);在高、中分化间及不同性别、年龄、民族中无统计学意义。Cox-2和Ki-67在MEC中表达正相关(rs=0.788,P<0.01)。结论涎腺黏液表皮样癌Cox-2、Ki-67的表达与组织病理分型有关,可能参与MEC的发生、发展过程,二者协同表达越高越容易发生浸润和远处转移;联合检测Cox-2、Ki-67可能有助于MEC的预后判断。
Objective To detect the expression of Cox-2 and Ki-67 in mucoepidermoid carcinoma (MEC) and normal salivary gland (NSG), and to provide a screening marker for the prognosis of MEC. Methods The expressions of Cox-2 and Ki-67 in 11 cases of NSG and 34 cases of MEC were detected by immunohistochemical staining (PV-6001 two-step method). The results were analyzed by SPSS 16.0 software. Results The expressions of Cox-2 in MEC and NSG were significantly different (P <0.01). The expression of Cox-2 in different pathological types (high, moderate, poorly differentiated) (P <0.05, P <0.01), but there was no significant difference in different gender, age, ethnicity and location. There was significant difference between the expression of Ki-67 in MEC and NSG (P <0.01), and there was significant difference in metastasis between high, moderate and poorly differentiated, P <0.01). There was no significant difference between the high and middle differentials and between different genders, ages and ethnic groups. Cox-2 and Ki-67 were positively correlated in MEC (rs = 0.788, P <0.01). Conclusions The expressions of Cox-2 and Ki-67 in mucoepidermoid carcinoma of salivary glands are related to the histopathological classification and may be involved in the occurrence and development of MEC. The higher the co-expression of Cox-2 and Ki-67, the higher the co-expression is, the more prone to infiltration and distant metastasis. -2, Ki-67 may contribute to the prognosis of MEC.