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以大豆胞囊线虫3号生理小种抗性品种灰皮支黑豆(ZDD2315)和感病品种辽豆15为材料,室内人工接种大豆胞囊线虫,检测抗、感品种侵染后二龄幼虫数量变化及根内活性氧变化,利用Real-Time Quantitative PCR分析了在大豆胞囊线虫3号生理小种互作下谷胱甘肽代谢途径相关基因在抗感大豆品种根内的相对表达量变化情况。结果表明:接种后第5天,抗感品种根内幼虫的数量大致相同;第15天,在灰皮支黑豆中,根内线虫幼虫数量减少;辽豆15根内H2O2含量始终略高于灰皮支黑豆;大豆胞囊线虫侵染后,谷氨酰胺半胱氨酸连接酶基因接种后3个时期的表达均上调;而谷胱甘肽合成酶基因在感病品种和抗病品种中处理组呈现了不同的表达趋势,尤其在接种15 d,在抗病品种灰皮支黑豆中表达下调,而在感病对照中表达上调;H型谷胱甘肽合成酶基因在抗性品种灰皮支黑豆中下调,说明谷胱甘肽相关基因的减少和下调在抗病品种灰皮支黑豆对大豆胞囊线虫的抗性呈正相关,导致大豆根系的氧化性增强,从而不利于大豆胞囊线虫的定殖和发育。
Soybean cyst nematode No. 3 physiological races gray pea black beans (ZDD2315) and susceptible varieties of Liaodou 15 as materials, indoor artificial inoculation Soybean cyst nematode to detect anti-susceptible varieties of second instar larvae after infection Changes in the root and intracellular reactive oxygen species, using Real-Time Quantitative PCR Analysis of soybean cyst Nematode physiological interactions under the interaction of glutathione-related genes in resistant roots of soybean varieties relative expression changes. The results showed that on the fifth day after inoculation, the number of root-knot larvae in resistant cultivars was approximately the same. On the fifteenth day, Black pea legumes; the expression of glutamine cysteine ligase gene was up-regulated at three stages after inoculation of Soybean cyst nematode, while the glutathione synthetase gene was treated in susceptible and resistant varieties Group showed different expression trends, especially in 15 d after inoculation, the expression was down-regulated in the disease-resistant cultivar Pinus sibirica and was up-regulated in the susceptible control. The H-glutathione synthetase gene Black beans, down regulation, indicating reduction and down-regulation of glutathione-related genes in the resistant varieties gray-backed black beans on soybean cyst nematode resistance was positive, leading to increased oxidation of soybean root, which is not conducive to soybean cyst nematode Colonization and development.