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目的探讨多位点序列分析技术在腹泻病原空肠弯曲菌分子溯源方面的运用。方法用多位点序列分析(MLST)对上海市浦东新区腹泻病原监测中的70株阳性空肠弯曲菌菌株的7个管家基因进行扩增、测序并上传至MLST数据库确定基因序列型(ST型),并运用Bio Numerics 7.1构建遗传进化树和聚类图进行亲缘性分析。结果 70株空肠弯曲菌分为52种已知ST型,分属13个同源谱系,ST353同源复合体占了流行株的26%。从70株分型结果中能找到聚集性病例。结论运用MLST技术能在分子水平上对空肠弯曲菌进行分型,并能直观表现菌株间的相互关系,从散发病例中找到聚集特征,为疾病的控制提供依据。
Objective To explore the application of multilocus sequence analysis in the molecular trace of Campylobacter jejuni in diarrhea. Methods Seven housekeeping genes of 70 positive strains of Campylobacter jejuni in the Pudong New Area of Shanghai were amplified by multilocus sequence analysis (MLST), sequenced and uploaded to the MLST database to identify the gene sequence (ST type) Bioinmerics 7.1 was used to construct phylogenetic tree and cluster graph for phylogenetic analysis. Results 70 strains of Campylobacter jejuni were divided into 52 known ST genotypes, belonging to 13 homologous genealogies, and ST353 homologous complex accounting for 26% of the epidemic strains. Aggregated cases can be found from 70 typing results. Conclusions MLST technique can be used to classify Campylobacter jejuni at the molecular level, and can intuitively show the interrelationship among strains. The clustering characteristics can be found from sporadic cases and provide basis for disease control.