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1 材料 中药黄芪自福建省中医学院国医堂购得,经鉴定为豆科多年生草本植物黄芪[Astragalus mem-branaceus(Fisch ge]的根。水煎浓缩至相当每ml含生药0.12g、0.2g、0.5g、1g、2g5个浓度。AFB_1为sigma公司产品,溶剂为二甲基亚砜(DMSO,上海金山化工厂)由福建省卫生防疫站分装成1mg/ml安瓿,低温避光保存,用时以DMSO稀释至相应浓度。实验动物由福建中医学院实验动物中心提供,昆明种小白鼠56只,体重18～22g,雌雄各半,按完全随机区组分成7组。设AFB_1阳性对照组,DMSO溶剂对照组和5个中药剂量组(2.5g/kg、5g/kg、10g/kg、20g/kg、40g/kg组)。每组8只,分为2笼,25℃按清洁级标准饲养。
1 The traditional Chinese medicine Astragalus membranaceus was purchased from the Department of Traditional Chinese Medicine of Fujian Provincial Traditional Chinese Medical College and was identified as the root of Astragalus mem-branaceus (Fisch ge), a perennial herb of the family Leguminosae. It was decocted to a concentration of 0.12g, 0.2g per ml of crude drug. 0.5g, 1g, 2g, 5 concentrations AFB_1 is a sigma product, and the solvent is dimethyl sulfoxide (DMSO, Shanghai Jinshan Chemical Plant), which is subdivided into 1mg/ml ampules by Fujian Sanitary and Epidemic Prevention Station, and stored at low temperature and protected from light. Diluted with DMSO to the corresponding concentration.Experimental animals were provided by Experimental Animal Center of Fujian College of Traditional Chinese Medicine, 56 Kunming mice, weighing 18-22g, male and female, divided into 7 groups according to completely randomized areas.Set AFB 1 positive control group, DMSO Solvent control group and 5 Chinese medicine dose groups (2.5g/kg, 5g/kg, 10g/kg, 20g/kg, 40g/kg groups), 8 in each group, divided into 2 cages, reared at 25°C according to clean grade standards .