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目的:探讨研究葛根素、盐酸小檗碱对大鼠脑组织和血浆中苯巴比妥浓度的影响。方法:120只SD大鼠随机分为空白对照1(苯巴比妥、8 mg·kg~(-1)、ig)、2(苯巴比妥、6.7 mg·kg~(-1)、iv)组、阳性对照(维拉帕米、10.3 mg·kg~(-1)、ig)1、2组、葛根素(150 mg·kg~(-1)、ig)1、2组、盐酸小檗碱(150 mg·kg~(-1)、ig)1、2组共8组,分别给予相应药物后,阳性对照1组、葛根素1组及盐酸小檗碱1组按8 mg·kg~(-1) ig给予苯巴比妥,阳性对照2组、葛根素2组及盐酸小檗碱2组按6.7 mg·kg~(-1) iv给予苯巴比妥,分别在给药后2,4,6 h处死大鼠,取脑、血浆制备样品,采用高效液相色谱法,测定在2,4,6 h各组大鼠脑组织和血浆中苯巴比妥浓度的变化。结果:与对应空白对照组相比,阳性对照组、葛根素组、盐酸小檗碱组在2 h时大鼠脑组织中苯巴比妥浓度无明显变化(P>0.05),但在4 h和6 h时能显著增加大鼠脑组织中苯巴比妥浓度,差异具有统计学意义(P<0.05),血浆中苯巴比妥浓度在监测各时段均无明显变化(P>0.05)。与对应阳性对照组比,葛根素组、盐酸小檗碱组均无显著差异(P>0.05)。结论:葛根素、盐酸小檗碱可以增加大鼠脑组织中苯巴比妥的浓度,对血浆中苯巴比妥浓度影响较小。
Objective: To investigate the effects of puerarin and berberine on the concentration of phenobarbital in rat brain and plasma. Methods: One hundred and twenty SD rats were randomly divided into control group 1 (phenobarbital, 8 mg · kg -1, ig), 2 (phenobarbital, 6.7 mg · kg -1, iv ), Positive control (verapamil, 10.3 mg · kg -1, ig), group 1,2, puerarin (150 mg · kg -1, ig) (150 mg · kg -1, ig), 1 group and 8 groups. After given the corresponding drugs, the positive control group 1, puerarin group 1 and berberine hydrochloride group 1 were treated with 8 mg · kg -1 ~ (-1) ig given phenobarbital, positive control group 2, puerarin group 2 and berberine hydrochloride group 2 were treated with 6.7 mg · kg -1 iv phenobarbital, respectively, after administration The rats were sacrificed at 2, 4 and 6 h, and the brain and plasma samples were prepared. The concentrations of phenobarbital in the brain and plasma of rats in each group were determined by HPLC at 2,4,6 h. Results: Compared with the blank control group, the concentration of phenobarbital in the brain tissue of the positive control group, the puerarin group and the berberine group did not change significantly at 2 h (P> 0.05), but at 4 h And 6 h significantly increased the concentration of phenobarbital in rat brain tissue, the difference was statistically significant (P <0.05), phenobarbital plasma concentrations in the monitoring of each period had no significant change (P> 0.05). Compared with the corresponding positive control group, there was no significant difference between puerarin group and berberine group (P> 0.05). Conclusion: Puerarin and berberine can increase the concentration of phenobarbital in rat brain, and have little effect on the concentration of phenobarbital in plasma.