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目的:研究灵芝孢子粉对2型糖尿病大鼠附睾组织中细胞色素C(Cyt-C)、线粒体Ca2+的影响。方法:青春期Wistar大鼠50只,大鼠尾静脉一次性注射2%链脲佐菌素(STZ)或柠檬酸-柠檬酸钠缓冲液制备2型糖尿病大鼠模型和对照组模型。糖尿病大鼠模型随机分模型组和灵芝组,每组20只,分别给以高脂高糖饮食、高脂高糖+灵芝孢子粉[250mg/(kg.d)],对照组10只,正常饮食。10周后,取双侧附睾,检测附睾细胞线粒体Ca2+、Cyt-C含量。结果:2型糖尿病模型制备成功,模型组附睾细胞线粒体Ca2+含量[(3.279±0.502)mg/L]明显高于对照组[(2.606±0.048)mg/L,P<0.01],灵芝组[(2.693±0.196)mg/L]明显低于模型组(P<0.05),与对照组差异无显著性(P>0.05)。模型组线粒体Cyt-C含量[(3.213±1.511)μmol/L]明显少于对照组[(5.688±1.679)μmol/L,P<0.05],胞质Cyt-C含量[(2.484±0.661)μmol/L]明显高于对照组[(1.574±0.329)μmol/L,P<0.01];灵芝组线粒体Cyt-C含量[(5.258±1.560)μmol/L]高于模型组,但差异无显著性(P>0.05),胞质Cyt-C含量[(1.727±0.396μmol/L]明显低于模型组(P<0.05),与对照组差异无显著性(P>0.05)。结论:2型糖尿病大鼠附睾细胞钙稳态失衡、线粒体有损伤,附睾细胞可能存在细胞凋亡过度。灵芝孢子粉在糖尿病状态下,对附睾组织有保护作用或有对抗细胞凋亡作用。
Objective: To study the effects of Ganoderma lucidum spores on cytochrome C (Cyt-C) and mitochondrial Ca2+ in the epididymis of type 2 diabetic rats. METHODS: Fifty puberty Wistar rats and one rat tail vein were injected with 2% streptozotocin (STZ) or citric acid-sodium citrate buffer to prepare type 2 diabetic rat model and control group model. Diabetic rat models were randomly divided into model group and Ganoderma lucidum group, and 20 rats in each group were given high-fat high-glucose diet, high-fat high-glucose + Ganoderma lucidum spore powder [250 mg/(kg.d)], and 10 rats in the control group. diet. After 10 weeks, the bilateral epididymis was taken and the mitochondrial Ca2+ and Cyt-C contents of epididymal cells were measured. Results: The type 2 diabetic model was successfully prepared. The mitochondrial Ca2+ content of the epididymis in the model group [(3.279±0.502) mg/L] was significantly higher than that of the control group [(2.606±0.048) mg/L, P<0.01]. The Ganoderma lucidum group [( 2.693±0.196) mg/L] was significantly lower than the model group (P<0.05), and there was no significant difference from the control group (P>0.05). The mitochondrial Cyt-C content in model group [(3.213±1.511) μmol/L] was significantly lower than that in control group [(5.688±1.679) μmol/L, P<0.05], cytoplasmic Cyt-C content [(2.484±0.661) μmol /L] was significantly higher than the control group [(1.574±0.329) μmol/L, P<0.01]; Ganoderma mitochondria Cyt-C content [(5.258±1.560) μmol/L] was higher than the model group, but the difference was not significant. (P>0.05), cytoplasmic Cyt-C content [(1.727±0.396 μmol/L) was significantly lower than the model group (P<0.05), and there was no significant difference with the control group (P>0.05).Conclusion: Type 2 diabetes mellitus In the rat epididymis, calcium homeostasis, mitochondrial damage, epididymal cells may have excessive apoptosis, Ganoderma lucidum spore powder has protective effect on epididymis tissue or anti-apoptotic effect in diabetes.