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国内测定肿瘤免疫、细胞免疫功能以及在教学实验等方面所用的MIF试验,其巨噬细胞大多都是从动物腹腔中分离。每次实验必须宰杀动物以分离巨噬细胞,既耗经济,又麻烦。测定MIF时一般常用毛细管法(间接法、直接法)、琼脂糖平板打孔法、琼脂糖微滴法、显微镜活动摄影技术~([1])、~8H胸腺核苷标记指示细胞等~([2])。但使用常规MIF测定法,仍然不能解决大量标本检测。我们试用传代的U-937株代替豚鼠腹腔渗出细胞(PEC)做MIF试验,并用ODMA对MIF测定,在2分钟内可测定96标本,其敏感性,快速性均优于毛细管法。
In domestic MIF assays for measuring tumor immunity, cellular immune function, and teaching experiments, macrophages are mostly isolated from the abdominal cavity of animals. Every experiment must kill animals to isolate macrophages, which is costly and troublesome. For the determination of MIF, capillary methods (indirect method, direct method), agarose plate punching method, agarose microdroplet method, microscope photography technique ([1]), ~8H thymidine labeling cell, etc. are generally used. [2]). However, the use of conventional MIF assays still cannot resolve a large number of specimen tests. We tested the passaged U-937 strain in place of guinea pig peritoneal exudate cells (PEC) for MIF assay, and used ODMA for MIF assay. 96 specimens could be measured within 2 minutes. The sensitivity and rapidity were better than capillary method.