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目的:观察红芪水提物对ALI大鼠肺组织形态以及NF-κBp65信号通路的影响,探讨其对ALI的治疗作用及相关机制。方法:SPF级Wistar大鼠60只,随机分为正常对照组、模型组、红芪高剂量组、红芪中剂量组、红芪低剂量组和地塞米松组。腹腔内注射内毒素5 m L/kg建立大鼠模型,光学显微镜观察肺部组织病理形态学变化,计算肺系数,实时荧光定量PCR检测肺组织中NF-κBp65(rela)m RNA的表达水平。结果:红芪高、中剂量组肺系数显著降低(P<0.01);红芪各治疗组肺组织病理损伤明显改善;红芪能明显降低NF-κBp65(rela)m RNA的表达量。结论:红芪能明显减轻内毒素所致急性肺损伤的炎症反应,其药理作用机制可能与抑制NF-κB信号通路的活化有关。
Objective: To observe the effects of extract of Radix Hedysari on lung morphology and NF-κBp65 signal pathway in ALI rats and to explore its therapeutic effect on ALI and related mechanisms. Methods: Sixty SPF Wistar rats were randomly divided into normal control group, model group, high dose of Radix Astragali, medium dose of Radix Hedysari, low dose of Radix Hedysari and dexamethasone group. The rat model was established by intraperitoneal injection of endotoxin 5 m L / kg. The pathological changes of lung tissue were observed with light microscope. The lung coefficient was calculated. The expression of NF-κBp65 (rela) m RNA in lung tissue was detected by real-time fluorescence quantitative PCR. Results: The lung coefficient of high and middle dose of Astragalus mongholicus decreased significantly (P <0.01), and the pathological changes of lung tissue of Hedysari were significantly improved. Hedysari can obviously decrease the expression of NF-κBp65 (rela) m RNA. Conclusion: Radix Astragali can significantly reduce the inflammatory reaction induced by lipopolysaccharide (LPS) -induced acute lung injury induced by endotoxin. The pharmacological mechanism may be related to the inhibition of the activation of NF-κB signaling pathway.