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目的:建立高效液相色谱法同时测定活血消肿丸中苦杏仁苷、芍药苷、阿魏酸、欧前胡素和山奈素含量的方法。方法:采用Waters Symmetry C18色谱柱,流动相为甲醇(A)和质量分数为0.1%的磷酸水溶液(B),梯度洗脱;柱温:30℃;流速:1.0ml·min-1;检测波长:苦杏仁苷和欧前胡素为210 nm,芍药苷为230 nm,阿魏酸为300 nm,山奈素为367 nm。结果:苦杏仁苷、芍药苷、阿魏酸、欧前胡素和山奈素的线性范围分别为10.66~533μg·ml-1(r=0.999 7),19.80~990μg·ml-1(r=0.999 8),4.04~202μg·ml-1(r=0.999 5),1.08~54μg·ml-1(r=0.999 5)和2.10~105μg·ml-1(r=0.999 6),5种成分的平均加样回收率(n=6)分别为99.3%,98.4%,99.0%,98.2%和99.0%,RSD分别为1.3%,1.1%,0.9%,1.0%和1.1%。结论:本法结果准确,重现性好,可用于活血消肿丸的质量控制。
Objective: To establish a method for the simultaneous determination of amygdalin, paeoniflorin, ferulic acid, imperatorin and kaempferol in Huoxuexiao Pill by HPLC. Methods: A Waters Symmetry C18 column was used. The mobile phase consisted of methanol (A) and 0.1% phosphoric acid (B) with gradient elution. The column temperature was 30 ℃ and the flow rate was 1.0 ml · min -1. The detection wavelength : 210 nm for amygdalin and imperatorin, 230 nm for paeoniflorin, 300 nm for ferulic acid and 367 nm for kaempferol. RESULTS: The linear ranges of amygdalin, paeoniflorin, ferulic acid, imperatorin and kaempferol were 10.66-553μg · ml-1 (r = 0.999 7) and 19.80-990μg · ml-1 (r = 0.999 (R = 0.999 5), 1.08-54 μg · ml-1 (r = 0.999 5) and 2.10-105 μg · ml-1 (r = 0.999 6) The recoveries (n = 6) were 99.3%, 98.4%, 99.0%, 98.2% and 99.0%, respectively, with RSDs of 1.3%, 1.1%, 0.9%, 1.0% and 1.1%, respectively. Conclusion: The method is accurate and reproducible, and can be used for quality control of Huoxue Xiaoying Pills.