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利用鲎试剂(鲎血细胞溶解物)检测微量细菌内毒素的鲎试验法,在我国引起越来越多的医药卫生工作者的重视,不少单位已经成功地制备了鲎试剂,并且用于样品检测。然而普遍使用的检测方法是试管法。这种方法虽然对于强阳性反应(形成固态凝胶)和阴性反应(无变化)容易判断,但对于区分弱阳性和阴性反应,在判断上就有一定困难。此外,这种方法要消耗较多的鲎试剂(每一试管0.1～0.2ml)。1974年 Frauch 首先提出微量鲎试验方法——载玻片实验。近来日本学者 Okuguc-hi S,在 Frauch 提出的方法基础上,进一步改进,增加了一步染色的操作,从而使载玻片
Laboratory tests for the detection of trace endotoxin in bacteria by limulus amebocyte lysate have drawn the attention of more and more medical and hygienic workers in our country. Many laboratories have successfully prepared limulus reagents and used for the detection of samples . However, the commonly used test is the test tube method. Although this method for the strong positive reaction (the formation of solid gel) and negative reactions (no change) easy to judge, but for the distinction between weak positive and negative reactions, there are some judgments in the difficulty. In addition, this method to consume more 鲎 reagent (each test tube 0.1 ~ 0.2ml). In 1974, Frauch first proposed the microtiter method - slide experiment. Recently, Japanese scholar Okugu-hi S, based on the method proposed by Frauch, further improved the operation of adding one-step staining so that the slide