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本文报告了用抗日本血吸虫肠相关趋阴极抗原单克隆抗体致敏血球建立反问间接血凝试验,对66例急性血吸虫病,123例慢性血吸虫病,57例肝吸虫病,54例钩虫病和82例非疫区健康人血清的检测效果。结果显示,该方法检测急、慢性血吸虫病血清的敏感性分别达到98.5%。和81.3%,与肝吸虫病血清交叉反应率为10.5%,与钩虫病血清未见交叉反应,与非疫区健康人血清仅有1.2%的假阳性巨应。与平行对照的McAb-Dot-ELISA检测结果相比,两者在检测敏感性和特异性上差别均无显著意义,具有较高符合率,表明McAb-RIHA试验是一种诊断效率和可靠性较高的检测日本血吸虫病血清循环抗原的方法,尤其适宜基层和现场应用。
This article reports the establishment of a retrospective indirect hemagglutination test using monoclonal antibodies against Schistosoma japonicum-associated cathode antigen to establish a retrospective indirect hemagglutination test. Sixty-six cases of acute schistosomiasis, 123 cases of chronic schistosomiasis, 57 cases of hepaticosis, 54 cases of hookworm and 82 cases of pest free area of healthy human serum test results. The results showed that the method of detection of acute and chronic schistosomiasis serum sensitivity reached 98.5%. And 81.3%, respectively. The cross-reactivity with hepatic clonorchiasis was 10.5%. No cross-reaction was found with the serum of hookworm and only 1.2% Compared with the results of parallel control McAb-Dot-ELISA, there was no significant difference between the two in the detection sensitivity and specificity, with a high coincidence rate, indicating that McAb RIHA test is a diagnostic efficiency and reliability than High detection of Schistosoma japonicum serum circulating antigen method, especially suitable for grassroots and field applications.