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目的:观察华蟾素对白血病细胞的凋亡诱导作用并探讨其机制。方法:用 MTT 法观察华蟾素对 HL-60细胞的生长抑制作用,通过形态学方法、DNA 电泳、流式细胞仪技术、Annexin V 标记法检测华蟾素作用后 HL-60细胞的凋亡发生情况,通过罗丹明染色检测华蟾素作用后 HL-60细胞线粒体跨膜电位的变化。结果:华蟾素作用后 HL-60细胞线粒体跨膜电位下降并发生调亡,在0.0625～0.5 μg/ml 的浓度范围内具有一定的量效关系。结论:华蟾素对白血病细胞具有凋亡诱导作用,其机制与破坏线粒体的功能有关。
Objective: To observe the apoptosis-inducing effect of Cinobufacini on leukemia cells and to explore its mechanism. METHODS: The growth inhibitory effect of Cinobufacini on HL-60 cells was observed by MTT assay. Apoptosis of HL-60 cells was detected by morphological method, DNA electrophoresis, flow cytometry, and Annexin V labeling assay. The occurrence of HL-60 cell mitochondrial transmembrane potential was detected by rhodamine staining. RESULTS: The mitochondrial transmembrane potential of HL-60 cells decreased and apoptotic after treated by Sinogeram, and there was a dose-effect relationship within the range of 0.0625-0.5 μg/ml. CONCLUSION: Cinobufacini has an apoptosis-inducing effect on leukemic cells, and its mechanism is related to the destruction of mitochondrial function.