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吸虫纲内幼虫的采集及其染色标本的制作均较困难,以致在教学与科学研究工作上对于幼虫外形与其内部构造的探讨均受了相当的影响,其中尤以日本血吸虫尾蚴染色标本的制作更属不易。因为日本血吸虫尾蚴虫体微小,尾部细长、分叉,且虫体与尾部间之接连部分又甚纤细,稍一不慎,尾部即与体部分离;其次,在固定时,尾部亦易卷曲,或分叉部分合并,甚难保持尾蚴之自然姿态。即或偶而固定得法,一旦经过染色、脱水、透明、封固等手续后,或残缺不全,或收缩变形,或在操作过程中随试剂之更换而流失,
It is difficult to collect the larvae in the Trematoda and produce the stained specimens so that the research on the larval appearance and the internal structure of the larvae in the teaching and scientific research are quite affected. Especially, the production of the stained specimens of Schistosoma japonicum Is not easy. Because of Schistosoma japonicum cercaria larvae tiny, slender tail, bifurcation, and the body and the tail between the successive part of the very slender, slightly careless, the tail that is separated from the body; Second, when fixed, the tail is also easy to curl , Or bifurcation part of the merger, it is difficult to maintain the natural cercariae posture. That is, or even fixed method, once after dyeing, dehydration, transparency, sealing and other procedures, or incomplete, or shrinkage, or in the course of the operation with the replacement of the loss,