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目的探讨磷酸酶基因(phosphataseandtensin homology deleted on chromosome ten,PTEN)在宫颈癌组织中的表达及其与宫颈癌临床生物学行为的关系。方法选取山东省潍坊市益都中心医院妇科2009年10月至2011年8月行门诊活检及手术切除的病理标本,其中宫颈癌组织59例、宫颈上皮内瘤变(CIN)组织21例及正常宫颈组织20例。采用免疫组织化学方法(SP法)检测各组织中PTEN表达情况,分析其表达情况与宫颈癌患者性别、年龄、分化程度、浸润深度、淋巴结转移、临床分期等临床生物学行为的关系。结果宫颈癌组织中PTEN的阳性表达率为38.98%(23/59),低于正常宫颈黏膜中的阳性表达率100.0%(20/20),二者比较差异有统计学意义(P<0.05)。宫颈癌组织中PTEN的表达随着癌灶的增大、分化程度的降低、浸润深度的加深、淋巴结的转移、临床分期的增加而明显降低。结论 (1)PTEN基因的失活参与了宫颈癌的发生。(2)PTEN的低表达预示着随着宫颈癌的进展和分化程度的降低,其侵袭和转移能力逐渐增强。
Objective To investigate the expression of phosphataseand Tensin homology deleted on chromosome ten (PTEN) in cervical cancer and its relationship with the clinical biological behavior of cervical cancer. Methods A total of 59 cases of cervical cancer, 21 cases of cervical intraepithelial neoplasia (CIN) and 21 normal controls were selected from the department of gynecology of Yidu Central Hospital, Weifang City, Shandong Province from October 2009 to August 2011. Cervical tissue in 20 cases. The expression of PTEN in each tissue was detected by immunohistochemistry (SP method), and the relationship between PTEN expression and clinical biological behaviors such as gender, age, differentiation, depth of invasion, lymph node metastasis and clinical stage were analyzed. Results The positive expression rate of PTEN in cervical cancer tissues was 38.98% (23/59), which was lower than that in normal cervical mucosa (100.0%, 20/20), the difference was statistically significant (P0.05) . The expression of PTEN in cervical cancer tissues decreased with the increase of tumor size, differentiation degree, depth of invasion, lymph node metastasis and clinical stage. Conclusions (1) Inactivation of PTEN gene is involved in the occurrence of cervical cancer. (2) The low expression of PTEN predicts that with the progress of cervical cancer and the degree of differentiation, the invasion and metastasis will gradually increase.