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目的:观察针灸对克罗恩病大鼠血清TNF-α、sTNFR-Ⅰ、sTNFR-Ⅱ的影响。方法:采用TNBS制备大鼠克罗恩病模型,随机分为模型组、隔药灸组、电针组,并与正常大鼠作对照,采用HE染色观察结肠黏膜组织病理学变化,应用ELISA方法检测血清TNF-α、sTNFR-Ⅰ、sTNFR-Ⅱ的含量。结果:与正常组比较,CD大鼠血清TNF-α显著升高、sTNFR-Ⅰ和sTNFR-Ⅱ无显著变化。经隔药灸和电针治疗后血清TNF-α水平显著降低,sTNFR-Ⅰ和sTNFR-Ⅱ无显著变化。CD大鼠结肠组织的炎症病变和异常的组织结构在隔药灸、电针治疗后得到明显改善。结论:隔药灸与电针均能够显著降低血清TNF-α的含量,该作用可能是隔药灸与电针治疗CD的作用机制之一。
Objective: To observe the effects of acupuncture and moxibustion on serum TNF-α, sTNFR-Ⅰ and sTNFR-Ⅱ in Crohn’s disease rats. Methods: The rat model of Crohn’s disease was established by TNBS. The model rats were randomly divided into model group, drug-moxibustion group and electro-acupuncture group, and compared with normal rats. The histopathological changes of colonic mucosa were observed by HE staining. Serum levels of TNF-α, sTNFR-Ⅰ and sTNFR-Ⅱ were detected. Results: Compared with the normal group, serum TNF-α in CD rats increased significantly, while sTNFR-Ⅰ and sTNFR-Ⅱ did not change significantly. The level of serum TNF-α was significantly decreased after treatment with moxibustion and electro-acupuncture, and no significant changes were found in sTNFR-Ⅰ and sTNFR-Ⅱ. Inflammatory lesions and abnormal tissue structures in the colon of CD rats were significantly improved after drug moxibustion and electroacupuncture treatment. Conclusion: Both drug-moxibustion and electro-acupuncture can significantly reduce the serum levels of TNF-α, which may be one of the mechanisms of drug-moxibustion and electro-acupuncture treatment of CD.