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我们以PEcob6含双拷贝乙型肝炎病毒的质粒为模板，进行聚合酶连反应，获取C区基因．平端连结克隆到PBKS+质粒中，选译出C区反义基因。再将C区反义基因亚克隆到PREP8质粒．构建成了乙型肝炎病毒C区反义基因的真核表达载体。
We use PEcob6 plasmid containing double copies of hepatitis B virus as a template for polymerase chain reaction to obtain the C region gene. The blunt end was cloned into PBKS + plasmid, and the C region antisense gene was selected. The C-region antisense gene was subcloned into PREP8 plasmid. Constructed into eukaryotic expression vector of antisense gene of hepatitis C virus C region.