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研究目的:柳树(Salix spp.)已广泛用于修复重金属污染,而且修复效率与树种及无性系有关。目前涉及柳树研究主要为铜(Cu)和锌(Zn)的毒性效应,而不是富集能力。本研究以中国常见的柳树无性系评价对Cu和Zn耐性与富集能力。创新要点:目前大多数研究仍集中Cu和Zn对柳树的毒性效应,尤其是Cu,对它们富集能力仍没有评价。中国为柳树的主要分布区。本文以12种优良的常见柳树无性系,通过温室营养液法评价对Cu和Zn耐性及富集潜力差异。利用柳树无性系的耐性与富集变异,旨在选择高耐性与高富集的无性系,提高环境修复效率和加快修复进程。研究方法:选择高生物量12种柳树无性系,采用温室营养液培养法添加Cu或Zn处理,测定生物量参数并计算耐性指数,估计耐性差异;用原子吸收法测定组织中金属浓度并计算金属位移系数,评价富集差异。重要结论:无性系对Cu和Zn耐性显著不同,生物量变化从无影响、抑制到促进生长。无性系对Cu耐性高于对Zn耐性,无性系对Zn富集变异幅度大于对Cu富集变异幅度。Cu主要富集于根部,而Zn较易运输到地上部分。总之测定的大部分柳树无性系为Cu与Zn高富集植物,显示出较高的植物提取能力。
Research purposes: Willix (Salix spp.) Has been widely used to repair heavy metal pollution, and the restoration efficiency and tree species and clones. The toxic effects currently involved in willow research are mainly copper (Cu) and zinc (Zn), rather than enrichment. In this study, tolerance and enrichment of Cu and Zn were evaluated by the common willow clones in China. Innovative Points: Most studies so far focus on the toxic effects of Cu and Zn on willow trees, especially Cu, which are still not evaluated for their enrichment capacity. China is the main distribution area of willow. In this paper, twelve elite common willow clones were used to evaluate their tolerance to Cu and Zn and their potential for enrichment by greenhouse nutrient solution method. With the tolerance and enrichment variation of willow clones, the aim was to select clones with high tolerance and high enrichment, to improve the efficiency of environmental remediation and speed up the process of remediation. Methods: Twelve willow clones with high biomass were selected. Cu or Zn was added to the greenhouse culture solution to measure the biomass parameters, and the tolerance index was calculated to evaluate the tolerance. The metal concentration in the tissues was determined by atomic absorption spectrometry Displacement coefficient, evaluation of enrichment differences. Important Conclusions: Clones have significantly different tolerance to Cu and Zn, with no change in biomass, inhibition to growth. The tolerance of clones to Cu was higher than that of Zn, and the variation of Zn enrichment in clones was greater than that of Cu enrichment. Cu is mainly concentrated in the roots, whereas Zn is easier to transport to aboveground parts. In conclusion, most of the willow clones determined to be Cu and Zn highly enriched plants showed high plant extractability.