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目的:探讨臭氧水关节腔注射治疗膝关节骨关节炎（knee osteoarthritis，KOA）对关节软骨损伤的修复作用及其内在的修复机制。方法:将48只SPF级SD大鼠随机分为空白组、模型组、生理盐水对照组和臭氧水组共4组，每组各12只。除空白组外，余各组采用木瓜蛋白酶关节腔注射建立KOA模型。取关节软骨行HE染色确认造模成功后，臭氧水组和生理盐水组分别行臭氧水和生理盐水关节注射治疗，每周1次，连续3周，空白组和模型组则不进行干预。分别于治疗前、后行大鼠膝关节行为学评分、治疗后行关节软骨表面大体评分、软骨组织HE染色及改良Mankin评分、Western blot和Rt-PCR分别检测软骨NF-κBp65（P65）、IKKβ及IκBα的mRNA和蛋白表达。结果:与模型组和生理盐水组相比，治疗后臭氧水组大鼠的膝关节行为学评分显著降低（均n P<0.05）；与模型组和生理盐水对照组相比，臭氧水组大鼠关节软骨表面大体评分、改良的Mankin评分均显著降低（均n P<0.05）；与模型组和生理盐水组相比，臭氧水组大鼠软骨组织P65和IKKβ的mRNA和蛋白表达明显降低（均n P<0.05），IκBα的mRNA和蛋白表达均明显升高（均n P<0.05）。n 结论:臭氧水关节腔注射治疗KOA能有效修复损伤的关节软骨，其修复机制可能是通过调控NF-κB信号通路而实现的。“,”Objective:To observe the repairing effect of ozone water injection in the articular cavity for the treatment of knee osteoarthritis (KOA) on articular cartilage and to explore its repair mechanism.Methods:48 rats were randomly divided into fourgroups, the normal, model, normal saline and ozone water group, each group had 12 rats. The rats were injectied into the joint cavity with papain to establish a KOA model other than the normal group. After confirming the success of the model, the ozone water group and normal saline group was treated with ozone water and normal saline injection into the joint cavity once a week for a total of 3 treatments, the normal group and the model group are all raised routinely. Before and after the treatment, the ratknee joint behavioral score MG score was conducted; after the treatment articular cartilage surface gross score, hematoxylin and eosin (HE) staining and modified Mankin score of articular cartilage pathological changes was measured, and Western blot and Rt-PCR to measure the level of protein and mRNA expression of NF-κB p65, IKKβ and IκBα in articular cartilage tissues.Results:Compared with before the treatment, the rat knee joint behavioral score of the ozone water group was significantly lower (all n P<0.05); after the treatment, the gross articular cartilage surface score and the modified Mankin score of the ozone water group were significantly reduced compared with the model and normal saline group (alln P<0.05); Compared with the model and normal saline group, the protein and mRNA expression levels of NF-κB p65 and IKKβ in the ozone water group are significantly lower (alln P<0.05), and the levels of IκBα are significantly higher (alln P<0.05).n Conclusion:Ozone water injection in the articular cavity can effectively repair damaged articular cartilage. The repair mechanism may be achieved by inhibiting the activation of NF-κB signaling pathway.