论文部分内容阅读
许多非感染性眼炎性疾病是根据病史和临床表现来分类的。近年来,它们的发病机理已不那么模糊了,看来,自身免疫性病变起着重要作用。为评价各种葡萄膜炎免疫活性细胞的特性和局部定位,已用针对各种炎症细胞的特殊膜抗原的单克隆抗体来进行免疫组织学研究。事实证明了炎症病灶占优势的细胞主要是 T-淋巴细胞,早期为辅助性/诱导性 T 细胞,而后期为抑制性/细胞毒性 T 细胞则相对增多。这一事实得到了实验性自身免疫性葡萄膜炎的相似发现的支持(和阐明),提示了免疫病理病变起始时 T-辅助性/诱导性细胞起主要作用,而抑制性/细胞毒性细胞则减弱—调节眼炎症活动。基于诊断的目的,我们在正常眼组织上滴几滴取自各种类型葡萄膜炎病人的血清进行染色;这样血清作为初次抗体用来检测针对正常组织里的光感受器和M(?)ller 细胞的自身免疫抗体。结果反映继发的或病理的现象与原发的致病因子稍有(或没有)直接关系。这一技术也利于诊断和鉴别某些不确定的葡萄膜炎。
Many non-infectious ocular inflammatory diseases are classified according to their medical history and clinical manifestations. In recent years, their pathogenesis has been less obscure, it seems, autoimmune lesions play an important role. To characterize various uveitis immunocompetent cells and to localize them, immunohistological studies have been performed with monoclonal antibodies against specific membrane antigens of various inflammatory cells. It turns out that predominantly inflammatory cells predominately T-lymphocytes, early helper / inducible T cells, and late suppressor / cytotoxic T cells. This fact is supported (and elucidated) by similar findings of experimental autoimmune uveitis suggesting that T-helper / induced cells play a major role at the start of immunopathological lesions, whereas inhibitory / cytotoxic cells Weakened - the regulation of ocular inflammatory activity. For diagnostic purposes, we stained a few drops of serum taken from various types of uveitis patients on normal ocular tissues; this serum was then used as an initial antibody to detect antibodies directed against photoreceptors and M (?) Ller cells in normal tissues Of autoimmune antibodies. The results reflect a secondary (or not) secondary or pathological phenomenon that is directly (or not) directly related to the primary virulence factor. This technique also facilitates the diagnosis and identification of some uncertain uveitis.