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本试验研究对连续辐照的大豆群体的种粒大小及比重进行长期选择的效果,并分离大粒型的高蛋白大豆品系。两个品种的种子都用8千拉德γ射线进行了急性照射。M_2代开始按种粒大小和比重进行选择。选出的种子再用1万拉德γ射线进行急性照射。上述处理在 M_3和 M_4代重复进行。各世代均测定了群体蛋白质含量及其他农艺性状。品种 Aobatsu 和 Tachisuzunari 在 M_5代,种粒比重高的小区与比重低的小区相比,蛋白质含量的最大差额分别为0.91%和2.25%。从 M_3、M_4、M_5代的结果可以看出,种子比重的选择对于从种粒中等大小的组内分离出高蛋白后代是较为有效的。另外,从 M_5代种粒大小的分布可明显地看出连续选择的效果。虽然试验还在进行之中,但通过连续辐照和种粒大小及比重的筛选,分离大粒型高蛋白品系将是可行的。
This study investigated the effects of long-term selection of the grain size and specific gravity of a continuously irradiated soybean population and the isolation of a large grain type high protein soybean line. Both varieties of seeds were irradiated acutely with 8 rads gamma rays. M_2 generation began to seed size and gravity selection. Eleven rads were selected for acute irradiation with gamma rays. The above processing is repeated in M_3 and M_4 generations. Population generations were assayed for protein content and other agronomic traits. Varieties Aobatsu and Tachisuzunari had the greatest differences in protein content between M_5 generation and those with higher specific gravity and those with lower specific gravity, respectively, of 0.91% and 2.25%, respectively. From the results of generations M_3, M_4 and M_5, it can be seen that the selection of seed specific gravity is more effective for the separation of high-protein offspring from the medium-sized groups. In addition, from the M_5 generation seed size distribution can clearly see the effect of continuous selection. Although the experiment is still under way, it will be feasible to isolate large-grain high-protein lines by continuous irradiation and screening of seed size and specific gravity.