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用酶免疫法测定 143例病毒性肝炎患者血清TGF - β1 水平 ,并与其中 87例慢性肝炎患者的肝组织病理对比。结果发现慢性肝炎及肝硬化患者血清TGF - β1 水平明显高于正常对照者 (P <0 .0 5 ) .与肝功能指标ALB呈中等程度的负相关 (r =- 0 .42 3,P =0 .0 0 0 ) ,与TBIL呈较弱的正相关 (r =0 .2 32 ,P =0 .0 0 7) ;肝组织炎症活动时 ,血清TGF - β1 水平明显升高 (P <0 .0 5 ) ,但两者之间无显著性相关 (P >0 .0 5 ) ;血清TGF - β1 水平与肝组织纤维化程度呈正相关 (r=0 .6 12 ,P <0 .0 5 ) ;肝硬化组血清TGF - β1 水平明显高于非肝硬化组 (P <0 0 5 ) ;血清TGF - β1 水平判断慢性肝炎患者S2 期以上肝纤维化及诊断肝硬化的ROC曲线下面积分别为 0 836和 0 82 8,截断值分别为 80ng ml和 12 0ng ml。结果表明动态监测血清TGF - β1 水平有助于了解肝纤维化的进展情况 ;血清TGF - β1 有助于判断慢性肝炎患者是否存在S2 期以上肝纤维化和诊断肝硬化 ,但与肝组织活检相比 ,其诊断价值有限
Serum levels of TGF - β1 in 143 patients with viral hepatitis were determined by enzyme - linked immunosorbent assay (ELISA), and were compared with those of 87 chronic hepatitis patients. The results showed that serum TGF - β1 levels in patients with chronic hepatitis and cirrhosis were significantly higher than those in controls (P <0.05), and negatively correlated with liver function index ALB (r = - 0.42 3, P = 0. 0 0 0), and had a weak positive correlation with TBIL (r = 0.322, P = 0.0007). Serum levels of TGF - β1 were significantly increased in inflammatory tissues of liver (P <0. 0.05), but there was no significant correlation between them (P> 0.05). The level of serum TGF - β1 was positively correlated with the degree of liver fibrosis (r = 0.62, P <0.05 ). The serum level of TGF - β1 in cirrhosis group was significantly higher than that in non - cirrhotic group (P0.05). The serum TGF - β1 level was used to determine the area under the ROC curve of liver fibrosis and cirrhosis at stage S2 in patients with chronic hepatitis 0 836 and 0 82 8, with cutoffs of 80 ng ml and 120 ng ml, respectively. The results indicate that the dynamic monitoring of serum TGF - β1 level helps to understand the progress of hepatic fibrosis. Serum TGF - β1 is helpful to determine whether there is S2 stage or more liver fibrosis and cirrhosis in patients with chronic hepatitis, but not with liver biopsy Than its diagnostic value is limited